CAJ | 학술논문

以中烟100为材料，研究了茉莉酸甲酯（MeJA）喷施后，烟草叶面腺毛密度类型、腺毛分泌物合成积累及叶片对蚜虫抗性的变化规律，探讨了烟草叶面腺毛的防御反应模式和作用机制。结果表明，MeJA处理后，烟草叶片对蚜虫的驱避作用明显增强，新生叶片叶面腺毛密度增加，尤其是长柄多细胞腺头腺毛密度的变化达到显著水平。GC/MS分析表明，MeJA处理使叶面分泌物含量增加，并在处理后第7天达到最大值。其中，西柏烷类二萜化合物含量增幅最大。但RT-PCR分析发现，西柏三烯一醇合成酶基因（CBT）、西柏三烯一醇羟化酶基因（CYP71D16）在转录水平上并没有明显变化，表明烟草叶面腺毛物质的合成和分泌可能具有复杂的转录后调控机制。
이중연100위재료，연구료말리산갑지（MeJA）분시후，연초협면선모밀도류형、선모분비물합성적루급협편대아충항성적변화규률，탐토료연초협면선모적방어반응모식화작용궤제。결과표명，MeJA처리후，연초협편대아충적구피작용명현증강，신생협편협면선모밀도증가，우기시장병다세포선두선모밀도적변화체도현저수평。GC/MS분석표명，MeJA처리사협면분비물함량증가，병재처리후제7천체도최대치。기중，서백완류이첩화합물함량증폭최대。단RT-PCR분석발현，서백삼희일순합성매기인（CBT）、서백삼희일순간화매기인（CYP71D16）재전록수평상병몰유명현변화，표명연초협면선모물질적합성화분비가능구유복잡적전록후조공궤제。
In order to explore the defense mode and mechanism of tobacco leaf trichome, Methyl jasmonate (MeJA) was choose to spray on tobacco seedling. After the treatment, trichome morphology, leaf surface chemical composition, gene expression and leaf resistance response to aphid were studied. Scanning electron microscope observation found that the trichome density of the new born leaves increased after MeJA treatment, especially the secreting type which has multicellular head. GC/MS analysis results showed that the content of leaf surface chemical substances increased, which reached the maximum value at the 7th day after treatment. Although the content of cembranoids, which was synthesized and secreted only by trichomes, changed greatly, the expression of its synthesis related genes, CBT and CYP71D16, did not show much difference during the MeJA treatment. It suggests that the complex posttranscriptional regulation mechanism in tobacco glandular hairs existed, which should be explored further.