CAJ | 학술논문

目的：：分离并鉴定一株多耐药鲍曼不动杆菌噬菌体，对其生物特性进行研究，为治疗多耐药鲍曼不动杆菌感染提供新的方法和实验依据。方法：以一株多耐药不动杆菌AB6为宿主菌，从医院废水中分离出噬菌体；用聚乙二醇6000对噬菌体进行浓缩和纯化，并就噬菌体的形态、一步生长曲线、裂解特性和不同保存条件对噬菌体活性的影响进行初步研究。结果：分离出一株噬菌体IME-AB6，电镜显示为肌尾噬菌体，其潜伏期为10 min，爆发期为40 min，爆发量160 pfu/cell；IME-AB6能迅速使菌液变清晰，温度灵敏性强，并且发现在4℃保存是一种方便高效的方法。结论：噬菌体IME-AB6是一株新的有独特特点的噬菌体，用聚乙二醇能很好地提高滴度，其潜伏期和爆发期短且杀菌效能强，性能稳定易保存，这对于噬菌体制剂的开发和耐药性鲍曼不动杆菌的防治具有潜在应用价值。
목적：：분리병감정일주다내약포만불동간균서균체，대기생물특성진행연구，위치료다내약포만불동간균감염제공신적방법화실험의거。방법：이일주다내약불동간균AB6위숙주균，종의원폐수중분리출서균체；용취을이순6000대서균체진행농축화순화，병취서균체적형태、일보생장곡선、렬해특성화불동보존조건대서균체활성적영향진행초보연구。결과：분리출일주서균체IME-AB6，전경현시위기미서균체，기잠복기위10 min，폭발기위40 min，폭발량160 pfu/cell；IME-AB6능신속사균액변청석，온도령민성강，병차발현재4℃보존시일충방편고효적방법。결론：서균체IME-AB6시일주신적유독특특점적서균체，용취을이순능흔호지제고적도，기잠복기화폭발기단차살균효능강，성능은정역보존，저대우서균체제제적개발화내약성포만불동간균적방치구유잠재응용개치。
Objective: To provide new strategy and experimental evidence for controlling multidrug-resistant Aci-netobacter baumannii(MDR-AB) infection, we isolated the bacteriophages of MDR-AB and investigated its biologi-cal characteristics. Methods: Bacteriophages were isolated from raw sewage with a MDR-AB strain MDR-AB6 as host cells. PEG6000 was used to concentrate and purify phage. Experiments were carried out to study the morphol-ogy and survey the one-step growth curve, cracking capacity and preservation methods of this phages. Results: A new phage was isolated and named as phage IME-AB6. Electron microscopy displays morphology resembling Myo-viridae family. It exhibited a short latent period(10 min) and also revealed that the outburst time was 40 min and the burst size was 160 pfu per cell. The phage has a robust bactericidal capacity and revealed excellent toler-ance to temperature. 4℃ preservation was proved to be a good and convenient method. Conclusion: The phage IME-AB6 is a new phage and has unique characteristics. The use of PEG method can improve the titer and puri-ty. It has a shorter latent and burst period, which implied that the IME-AB6 was more lytic. These studies will help to develop phage IME-AB6 as a promising therapeutic alternative for the future.