生物技术通讯
生物技術通訊
생물기술통신
LETTERS IN BIOTECHNOLOGY
2013年
5期
636-640
,共5页
刘甦苏%周舒雅%高正琴%左琴%岳秉飞%贺争鸣%刘佐民%张冰%范昌发
劉甦囌%週舒雅%高正琴%左琴%嶽秉飛%賀爭鳴%劉佐民%張冰%範昌髮
류소소%주서아%고정금%좌금%악병비%하쟁명%류좌민%장빙%범창발
C57-ras癌症小鼠模型%转基因c-Ha-ras%半定量PCR%荧光定量PCR%基因表达谱
C57-ras癌癥小鼠模型%轉基因c-Ha-ras%半定量PCR%熒光定量PCR%基因錶達譜
C57-ras암증소서모형%전기인c-Ha-ras%반정량PCR%형광정량PCR%기인표체보
C57-ras cancer transgenic mice%transgene c-Ha-ras%semi-quantitative RT-PCR%real-time RT-PCR%gene expression profile
目的::分析转基因c-Ha-ras在C57-ras癌症小鼠模型中的组织表达谱及时空表达差异。方法:利用半定量及荧光定量RT-PCR法,分析不同首建鼠系、不同周龄小鼠各脏器中转基因c-Ha-ras的表达。结果:转基因c-Ha-ras在心、肝等13种组织器官中均有表达,在肺脏中表达最高,在肝脏中表达最低,No.2、No.3和No.5等3个首建鼠均呈现相似的变化规律;转基因在No.5首建鼠中表达水平最高,而在同一个首建鼠系中,12周龄时表达高于8周龄和24周龄。结论:转基因c-Ha-ras在各脏器中能高效表达,并间接表明该转基因能稳定遗传,为C57-ras癌症小鼠模型用于新药临床前致癌性评价提了供理论支持。
目的::分析轉基因c-Ha-ras在C57-ras癌癥小鼠模型中的組織錶達譜及時空錶達差異。方法:利用半定量及熒光定量RT-PCR法,分析不同首建鼠繫、不同週齡小鼠各髒器中轉基因c-Ha-ras的錶達。結果:轉基因c-Ha-ras在心、肝等13種組織器官中均有錶達,在肺髒中錶達最高,在肝髒中錶達最低,No.2、No.3和No.5等3箇首建鼠均呈現相似的變化規律;轉基因在No.5首建鼠中錶達水平最高,而在同一箇首建鼠繫中,12週齡時錶達高于8週齡和24週齡。結論:轉基因c-Ha-ras在各髒器中能高效錶達,併間接錶明該轉基因能穩定遺傳,為C57-ras癌癥小鼠模型用于新藥臨床前緻癌性評價提瞭供理論支持。
목적::분석전기인c-Ha-ras재C57-ras암증소서모형중적조직표체보급시공표체차이。방법:이용반정량급형광정량RT-PCR법,분석불동수건서계、불동주령소서각장기중전기인c-Ha-ras적표체。결과:전기인c-Ha-ras재심、간등13충조직기관중균유표체,재폐장중표체최고,재간장중표체최저,No.2、No.3화No.5등3개수건서균정현상사적변화규률;전기인재No.5수건서중표체수평최고,이재동일개수건서계중,12주령시표체고우8주령화24주령。결론:전기인c-Ha-ras재각장기중능고효표체,병간접표명해전기인능은정유전,위C57-ras암증소서모형용우신약림상전치암성평개제료공이론지지。
Objective: To analyze gene expression profile and the time-space expression distribution of c-Ha-ras gene in C57-ras cancer transgenic mice. Methods:Primers were designed according to c-Ha-ras gene sequence, we analyzed expression profile in different founders and various tissues of C57-ras cancer transgenic mice by semi-quantitative RT-PCR and real-time RT-PCR method. Results:Analysis showed that c-Ha-ras had the high-est expressional level in lung, and lowest in liver. Similar change rule were found in No.2, No.3 and No.5 lines. In the aspect of expression in different time phase, comparison was done in the same line, 12-week C57-ras mice was higher than 8-week and 24-week. Among all the three founder lines, c-Ha-ras expressed highest in No.5. Conclusion:Transgenic c-Ha-ras expressed in the C57-ras cancer mice, which provided theoretical support for applying this mouse model in new drug preclinical carcinogenicity evaluation. Due to the highest expression in line No.5, it might be breeding and studied in carcinogenicity preferentially. Furthermore, since analysis was based on 12 generations of C57-ras mice, expression in various organs and different generations verified the stable hered-ity of c-Ha-ras gene.