CAJ | 학술논문

目的：：为了探讨与人血细胞相关的GATA1转录因子在实体肿瘤发生发展中的功能，构建GATA1的慢病毒干扰载体，并验证其敲低效果。方法：根据人GATA1的cDNA序列，设计含有小发卡结构的寡核苷酸序列，将其克隆到慢病毒表达载体上；将重组质粒转染人胚肾293T细胞，通过实时定量RT-PCR及Western印迹检测GATA1的表达水平。结果和结论：构建了具有明显干扰效果的GATA1基因的小干扰RNA（siRNA）慢病毒表达载体，能够有效抑制GATA1 mRNA和蛋白水平，为后续的GATA1生物学作用研究奠定了基础。
목적：：위료탐토여인혈세포상관적GATA1전록인자재실체종류발생발전중적공능，구건GATA1적만병독간우재체，병험증기고저효과。방법：근거인GATA1적cDNA서렬，설계함유소발잡결구적과핵감산서렬，장기극륭도만병독표체재체상；장중조질립전염인배신293T세포，통과실시정량RT-PCR급Western인적검측GATA1적표체수평。결과화결론：구건료구유명현간우효과적GATA1기인적소간우RNA（siRNA）만병독표체재체，능구유효억제GATA1 mRNA화단백수평，위후속적GATA1생물학작용연구전정료기출。
Objective: To study the biological function of human blood cells-associated GATA1 in the development and progression of solid tumor, the lentiviral vector for GATA1 small-interfering RNA(siRNA) was constructed and its knockdown effect was determined. Methods: GATA1 siRNA was designed and constructed based on human GATA1 cDNA sequence using a lentiviral vector. The expression of GATA1 was examined by real-time RT-PCR and Western blot analysis. Results & Conclusion: A lentivirus RNA interference vector targeting GATA1 gene was successfully constructed, which laid the foundation for further study of GATA1 function.