泸州医学院学报
瀘州醫學院學報
로주의학원학보
JOURNAL OF LUZHOU MEDICAL COLLEGE
2013年
5期
427-431
,共5页
魏嵋%王晓栋%肖斌%刘友平
魏嵋%王曉棟%肖斌%劉友平
위미%왕효동%초빈%류우평
慢性乙肝%中医证型%免疫反应%蛋白质组学
慢性乙肝%中醫證型%免疫反應%蛋白質組學
만성을간%중의증형%면역반응%단백질조학
Chronic hepatitis B%Traditional Chinese medical syndromes%Immune response%Proteomics
目的:通过分析慢性乙肝5种中医证型患者外周血浆蛋白质的差异表达程度,初步探索慢性乙肝各中医证型间免疫反应状况规律。方法:采用双向电泳技术结合质谱对慢性乙肝5种中医证型患者(各30例)及正常对照组(10例)的血浆全蛋白进行比较蛋白质组学分析,然后扩大样本量,选取其中与肝脏损伤及机体免疫功能相关的差异表达蛋白质进行ELISA检测其变化规律。结果:①比较蛋白质组学分析发现在5种慢性乙肝中医证型中均存在明显规律性差异表达的蛋白点共有5个:(haptoglobin,HPT)、免疫球蛋白J链(immunoglobulin J-chains, IGJ)、视黄醇结合蛋白(retinol binding protein, RBP)、C-反应蛋白(C-reactive protein,CRP)、玻连蛋白(vitronectin,VN)。②ELISA检测发现在5种中医证型中与肝脏功能相关的蛋白质---RBP、HPT较正常对照组下调与机体免疫功能相关的蛋白质---IGJ较正常对照组上调的高低顺序是一致的。结论:慢性乙肝中医证型演变过程与机体体液免疫状态之间存在正相关性,肝脏病情越严重的中医证型其机体免疫功能越紊乱。
目的:通過分析慢性乙肝5種中醫證型患者外週血漿蛋白質的差異錶達程度,初步探索慢性乙肝各中醫證型間免疫反應狀況規律。方法:採用雙嚮電泳技術結閤質譜對慢性乙肝5種中醫證型患者(各30例)及正常對照組(10例)的血漿全蛋白進行比較蛋白質組學分析,然後擴大樣本量,選取其中與肝髒損傷及機體免疫功能相關的差異錶達蛋白質進行ELISA檢測其變化規律。結果:①比較蛋白質組學分析髮現在5種慢性乙肝中醫證型中均存在明顯規律性差異錶達的蛋白點共有5箇:(haptoglobin,HPT)、免疫毬蛋白J鏈(immunoglobulin J-chains, IGJ)、視黃醇結閤蛋白(retinol binding protein, RBP)、C-反應蛋白(C-reactive protein,CRP)、玻連蛋白(vitronectin,VN)。②ELISA檢測髮現在5種中醫證型中與肝髒功能相關的蛋白質---RBP、HPT較正常對照組下調與機體免疫功能相關的蛋白質---IGJ較正常對照組上調的高低順序是一緻的。結論:慢性乙肝中醫證型縯變過程與機體體液免疫狀態之間存在正相關性,肝髒病情越嚴重的中醫證型其機體免疫功能越紊亂。
목적:통과분석만성을간5충중의증형환자외주혈장단백질적차이표체정도,초보탐색만성을간각중의증형간면역반응상황규률。방법:채용쌍향전영기술결합질보대만성을간5충중의증형환자(각30례)급정상대조조(10례)적혈장전단백진행비교단백질조학분석,연후확대양본량,선취기중여간장손상급궤체면역공능상관적차이표체단백질진행ELISA검측기변화규률。결과:①비교단백질조학분석발현재5충만성을간중의증형중균존재명현규률성차이표체적단백점공유5개:(haptoglobin,HPT)、면역구단백J련(immunoglobulin J-chains, IGJ)、시황순결합단백(retinol binding protein, RBP)、C-반응단백(C-reactive protein,CRP)、파련단백(vitronectin,VN)。②ELISA검측발현재5충중의증형중여간장공능상관적단백질---RBP、HPT교정상대조조하조여궤체면역공능상관적단백질---IGJ교정상대조조상조적고저순서시일치적。결론:만성을간중의증형연변과정여궤체체액면역상태지간존재정상관성,간장병정월엄중적중의증형기궤체면역공능월문란。
Objective: To explore the link between the immune response and the five types of traditional Chinese medicine(TCM)syndromes of patients with chronic hepatitis B(CHB)by proteomic analysis. Methods:Plasma was prepared from 150 cases of CHB patients with five different types of TCM syndromes and 10 cases of normal control.Two dimensional gel electrophoresis(2-DE)followed by matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS) was used to pick out important protein spots which had different expressions among different TCM syndromes and normal controls, and three of these differentially expressed proteins which related to liver injury and immune function were quantitatively analyzed by ELISA from more plasma samples. Results: Five differential proteins were: immunoglobulin J-chains (IGJ), C-reactive protein(CRP), haptoglobin(HPT), retinol binding protein (RBP)and vitronectin(VN). And the upregulation tendency of IGJ which related to the immune function were consistent with the downregulation tendency of HPT and RBP which related to liver injury. Conclusion: The humoral immunization play a very important role in the process of the five TCM syndromes of CHB and the stronger humoral immune response is, the more severe liver damage is.