兰州大学学报(自然科学版)
蘭州大學學報(自然科學版)
란주대학학보(자연과학판)
JOURNAL OF LANZHOU UNIVERSITY(NATURAL SCIENCES)
2013年
5期
688-692
,共5页
李巧峡%安珂%付亚娟%周琦%赵庆芳%杨宁%孙坤
李巧峽%安珂%付亞娟%週琦%趙慶芳%楊寧%孫坤
리교협%안가%부아연%주기%조경방%양저%손곤
阿魏酸%莴苣%幼根净生长值%有丝分裂指数%细胞周期
阿魏痠%萵苣%幼根淨生長值%有絲分裂指數%細胞週期
아위산%와거%유근정생장치%유사분렬지수%세포주기
ferulic acid%lettuce%pure growth of root%mitotic index%cell cycle
低浓度阿魏酸对莴苣幼根生长发育有一定的促进作用,当浓度为0.05,0.50 mmol/L时,幼根净生长值和有丝分裂指数相对较高,分别为11.47 mm,9.88%和11.09 mm,11.22%.在低浓度下,幼苗根尖细胞有丝分裂前期细胞比率较高,当浓度为0.05,0.50 mmol/L时,根尖细胞有丝分裂前期指数分别为6.57%,7.26%.而高浓度的阿魏酸对莴苣幼根生长发育有抑制作用,当浓度为1.00 mmol/L时,幼根净生长值和有丝分裂指数为1.26 mm,6.41%,有丝分裂前期指数为0.98%.用流式细胞仪检测莴苣根尖分生区细胞周期的变化,结果表明:1.00 mmol/L阿魏酸对莴苣幼苗根尖分生区细胞的细胞周期具有阻滞作用,主要阻滞了S期和G2期,且阻滞效应与阿魏酸的作用时间有关,处理时间越长则阻滞越明显.
低濃度阿魏痠對萵苣幼根生長髮育有一定的促進作用,噹濃度為0.05,0.50 mmol/L時,幼根淨生長值和有絲分裂指數相對較高,分彆為11.47 mm,9.88%和11.09 mm,11.22%.在低濃度下,幼苗根尖細胞有絲分裂前期細胞比率較高,噹濃度為0.05,0.50 mmol/L時,根尖細胞有絲分裂前期指數分彆為6.57%,7.26%.而高濃度的阿魏痠對萵苣幼根生長髮育有抑製作用,噹濃度為1.00 mmol/L時,幼根淨生長值和有絲分裂指數為1.26 mm,6.41%,有絲分裂前期指數為0.98%.用流式細胞儀檢測萵苣根尖分生區細胞週期的變化,結果錶明:1.00 mmol/L阿魏痠對萵苣幼苗根尖分生區細胞的細胞週期具有阻滯作用,主要阻滯瞭S期和G2期,且阻滯效應與阿魏痠的作用時間有關,處理時間越長則阻滯越明顯.
저농도아위산대와거유근생장발육유일정적촉진작용,당농도위0.05,0.50 mmol/L시,유근정생장치화유사분렬지수상대교고,분별위11.47 mm,9.88%화11.09 mm,11.22%.재저농도하,유묘근첨세포유사분렬전기세포비솔교고,당농도위0.05,0.50 mmol/L시,근첨세포유사분렬전기지수분별위6.57%,7.26%.이고농도적아위산대와거유근생장발육유억제작용,당농도위1.00 mmol/L시,유근정생장치화유사분렬지수위1.26 mm,6.41%,유사분렬전기지수위0.98%.용류식세포의검측와거근첨분생구세포주기적변화,결과표명:1.00 mmol/L아위산대와거유묘근첨분생구세포적세포주기구유조체작용,주요조체료S기화G2기,차조체효응여아위산적작용시간유관,처리시간월장칙조체월명현.
The growth of seedling root of lettuce was promoted at a lower concentration of ferulic acid. Pure growth of root and mitotic index were higher under treatment at 0.05, 0.50 mmol/L and their values were 11.47 mm, 9.88%and 11.09 mm, 11.22%respectively. Under the concentration of 0.05, 0.50 mmol/L, the mitosis prophase cell ratio of root tip was higher, and they were 6.57% and 7.26% respectively. But the growth of seedling root was significantly inhibited with the treatment of 1.00 mmol/L of ferulic acid, the pure growth of root and mitotic index were 1.26 mm and 6.41% respectively and the mitosis prophase cell ratio was 0.98%. Moreover, the cell cycle of root apex under the treatment at 1.00 mmol/L was detected by using flow cytometry. The results indicated that S phase and G2 phase in cell cycle were mainly blocked. The blocked effect was associated with the processing time of ferulic acid, and it became more severe with the increase of processing time.