CAJ | 학술논문

采用生物信息学方法对GenBank, SwissProt中的拟南芥、大豆、甘蓝型油菜、小麦等物种的乙酰辅酶A羧化酶核苷酸和氨基酸序列进行了比对分析,进而对其组成成分、理化性质、疏水性/亲水性、跨膜结构域、蛋白质二级结构、功能结构域、三级结构数进行了预测和分析.结果表明： ACC基因具有完整的开放阅读框,长约50683 bp,编码氨基酸相对分子质量为93.41 kD,理论等电点为7.27,是亲水性不稳定蛋白,二级结构均以α-螺旋和不规则卷曲为主要构件.通过进化树分析可分别将8种生物分为2组： A支包括小麦等7个物种,B支仅含甘蓝型油菜.
채용생물신식학방법대GenBank, SwissProt중적의남개、대두、감람형유채、소맥등물충적을선보매A최화매핵감산화안기산서렬진행료비대분석,진이대기조성성분、이화성질、소수성/친수성、과막결구역、단백질이급결구、공능결구역、삼급결구수진행료예측화분석.결과표명： ACC기인구유완정적개방열독광,장약50683 bp,편마안기산상대분자질량위93.41 kD,이론등전점위7.27,시친수성불은정단백,이급결구균이α-라선화불규칙권곡위주요구건.통과진화수분석가분별장8충생물분위2조： A지포괄소맥등7개물충,B지부함감람형유채.
The nucleic acid sequence and amino acid sequence of acetyl-CoA carboxylase (ACC) from Ara-bidopsis thaliana, Glycine max, Brassica napus, and Triticum aestivum, which were registered in GenBank and SwissProt, were analyzed and predicted with the tools of bioinformatics in the following main aspects, i.e. the composition of nucleic acid sequence and amino acid sequence, physical and chemical characters, hydrophobicity or hydrophilicity, transmembrane topologieal structure, secondary structure of protein, functional domains and tertiary structure. The results showed that ACC gene contained a complete open reading frame with length of 50 683 bp, the molecular weight of amino acids was 93.41 kD, the theoretical isoelectric point was 7.27. This was a hydrophilic and instable protein, in which alpha helix and random coil were the main components of all secondary structures. The eight species were divided into two groups by phylogenetic analysis, A branch including seven species including Triticum aestivum, and B branch only containing B. napus.