CAJ | 학술논문

群体结构是引起关联分析假阳性的最主要因素。本研究基于25个EST-SSRs位点，分析了283份橡胶树魏克汉种质群体的哈迪-温伯格平衡和群体分层情况。结果表明，在被检查的25个位点中13个位点偏离哈迪-温伯格平衡；在共祖系数为50%、60%、70%和80%时，283份种质中具有明确亚群归属的分别只有155份、110份、61份和22份，分别占总种质的54.78%、38.87%、21.55%和7.78%。同时，25个多态位点共形成了300个位点组合，20个位点组合(6.67%)呈显著的连锁不平衡状态，其中5个位点组合(1.67%)达到极显著连锁不平衡。由上可知283份橡胶树魏克汉种质所构成的群体具有一定的群体分层，但分化程度较小，可用于关联分析。
군체결구시인기관련분석가양성적최주요인소。본연구기우25개EST-SSRs위점，분석료283빈상효수위극한충질군체적합적-온백격평형화군체분층정황。결과표명，재피검사적25개위점중13개위점편리합적-온백격평형；재공조계수위50%、60%、70%화80%시，283빈충질중구유명학아군귀속적분별지유155빈、110빈、61빈화22빈，분별점총충질적54.78%、38.87%、21.55%화7.78%。동시，25개다태위점공형성료300개위점조합，20개위점조합(6.67%)정현저적련쇄불평형상태，기중5개위점조합(1.67%)체도겁현저련쇄불평형。유상가지283빈상효수위극한충질소구성적군체구유일정적군체분층，단분화정도교소，가용우관련분석。
Population stratification is key reason resulting in false association in association analysis. Analysis of Hardy-Weinberg equilibrium and population structure on 283 Wickham germplasm was carried out by 25 EST-SSRs. The result showed that 13 of 25 EST-SSRs showed that there were Hardy-Weinberg equilibrium, and 54.78%, 38.87%, 21.55%and 7.78%of entire Wickham germplasm could be of population stratification, additionally, 20 locus combinations (6.67%) of 300 EST-SSRs combinations-derived 25 EST-SSRs were significantly linkage dis-equilibrium. So there was genetic diversity of 283 Wickham germplasm but the level of genetic diversity was lower, the population could be used for association study.