CAJ | 학술논문

甲基磺酸乙酯(EMS)诱变育种有着诱变频率高、突变性状多及破坏性小等优点被广泛用于多种作物诱发突变育种。本研究利用不同浓度的EMS对不同品种的萝卜种子进行浸种处理后，发现EMS对萝卜的种子发芽率、萝卜苗根部长度及田间性状等指标上的诱变效应。研究结果表明随着EMS浓度(0.2%~2%)的增加，种子发芽率急剧降低且萝卜苗期根长明显变短，整体呈显著的抑制生长作用。不同品种间对EMS的敏感度依次顺序为短叶-13>夏抗40>春白11-58>双红一号。以半致死浓度为EMS浓度选择标准，确定了EMS处理不同品种萝卜间的适宜浓度为0.4%~0.6%。通过田间性状调查发现0.5%EMS处理短叶13萝卜种子下播大田后，相比对照处理品种表现子叶卷曲及撕裂、真叶增厚及黄化等情况，进一步表明EMS对短叶13萝卜存在的诱变效应。本研究确定了化学诱变剂EMS对萝卜诱变的半致死剂量，初步确认EMS对萝卜早期生长发育性状的诱变效应，将为后期的萝卜诱变育种研究提供研究材料和参考。
갑기광산을지(EMS)유변육충유착유변빈솔고、돌변성상다급파배성소등우점피엄범용우다충작물유발돌변육충。본연구이용불동농도적EMS대불동품충적라복충자진행침충처리후，발현EMS대라복적충자발아솔、라복묘근부장도급전간성상등지표상적유변효응。연구결과표명수착EMS농도(0.2%~2%)적증가，충자발아솔급극강저차라복묘기근장명현변단，정체정현저적억제생장작용。불동품충간대EMS적민감도의차순서위단협-13>하항40>춘백11-58>쌍홍일호。이반치사농도위EMS농도선택표준，학정료EMS처리불동품충라복간적괄의농도위0.4%~0.6%。통과전간성상조사발현0.5%EMS처리단협13라복충자하파대전후，상비대조처리품충표현자협권곡급시렬、진협증후급황화등정황，진일보표명EMS대단협13라복존재적유변효응。본연구학정료화학유변제EMS대라복유변적반치사제량，초보학인EMS대라복조기생장발육성상적유변효응，장위후기적라복유변육충연구제공연구재료화삼고。
Mutated breeding by ethylmethylsulfone (EMS) has several advantages:high mutation rate, much mutated phenotype and low destruction which has been widely applied for crop breeding. In this study, the germination ratio, root length and agronomic traits in field of mutated seeds were investigated. The results showed that germination ratio, root length and the leaf growth of radish were inhibited as the increasing concentration of EMS (from 0.2%to 2%) which indicated the inhibited effect of EMS to radish. Whereas the sensitivity to EMS was different among differ-ent radish cultivators:Duanye-13>Xiakang-40>Chunbai-11-85>Shuanghong No 1. Moreover, the suitable concentra-tion of EMS for radish was 0.4%~0.6%by the selection criteria of lethal concentration 50. The field traits were recorded after soaking seeds of Duanye-13 treated by 0.5%EMS. The abnormities from treated group were found widely which focused on cotyledon curling and tearing, true leaf thicken and epilation by compared with CK group. This study identified the dose of lethal concentration 50 for radish mutation by EMS and confirmed the mutagenic effect of EMS to radish in aspect of seed germination and leaf development which will provide support and refer-ence for radish breeding in future.