CAJ | 학술논문

MF11a为甘蔗糖蜜乙醇发酵野生型高产菌株MF1002的呼吸突变体，对糖分的利用能力显著高于MF1002。本文研究了这两菌株应激高糖胁迫的生理特性变化。结果表明，高糖培养条件下，MF11a菌株的生长和乙醇发酵受抑制的程度均明显低于MF1002，培养基的葡萄糖浓度为30%和40%时，其最大菌体密度、最高出芽率和乙醇浓度等已显著高于MF1002，表明MF11a较MF1002具有更强的高糖耐受能力。在30%葡萄糖的胁迫培养条件下，两菌株胞内的总超氧化物歧化酶(SOD)活力、过氧化氢酶活力、过氧化物酶活力，及它们细胞质和线粒体的ATP酶活力均显著上升，说明这五种酶均参与了两菌株的高糖胁迫反应。其中，MF11a的胞内过氧化氢酶活性、过氧化物酶活力、细胞质ATP酶活力在高糖胁迫下的上升幅度显著高于MF1002，表明这三种酶活力可能与MF11a菌株的高糖耐受能力有关，可作为该菌株进一步改造的指导指标。
MF11a위감자당밀을순발효야생형고산균주MF1002적호흡돌변체，대당분적이용능력현저고우MF1002。본문연구료저량균주응격고당협박적생리특성변화。결과표명，고당배양조건하，MF11a균주적생장화을순발효수억제적정도균명현저우MF1002，배양기적포도당농도위30%화40%시，기최대균체밀도、최고출아솔화을순농도등이현저고우MF1002，표명MF11a교MF1002구유경강적고당내수능력。재30%포도당적협박배양조건하，량균주포내적총초양화물기화매(SOD)활력、과양화경매활력、과양화물매활력，급타문세포질화선립체적ATP매활력균현저상승，설명저오충매균삼여료량균주적고당협박반응。기중，MF11a적포내과양화경매활성、과양화물매활력、세포질ATP매활력재고당협박하적상승폭도현저고우MF1002，표명저삼충매활력가능여MF11a균주적고당내수능력유관，가작위해균주진일보개조적지도지표。
The wild type Saccharomyces cerevisiae strain MF1002 that was a high yield strain of molasses ethanol fermentation, and its respiratory mutant MF11a of which the sugar utilizing capacity was significantly enhanced, were employed to investigate the physiologic response to the high sugar stress. When cultivated in the medium con-taining high concentration glucose, the growth and ethanol fermentation of MF1002 were repressed significantly stronger than MF11a by high sugar stress. With the glucose concentration in medium was increased to 30%and 40%, the maximum cell number, the maximum cell budding ratio and the ethanol fermentation concentration of MF11a were all significantly higher than that of MF1002, indicating that MF11a was stronger tolerance than MF1002 to high sugar stress. The enzymes activity assay exhibited that five enzyme activities of both strains, e.g. the superoxide dismutase (SOD) activity in cell extract, the catalase activity in cell extract, the peroxidase activity in cell extract, the ATPase activity in cytoplasm and the ATPase activity in mitochondria, were all significantly increased after the strains were cultivated in the medium containing 30%glucose for 24 h for high sugar stress, indicating these enzymes were involved in the response of both strains to high sugar stress. Of which, the increment of the catalase activity in cell extract, the peroxidase activity in cell extract and the ATPase activity in cytoplasm for MF11a was significant higher than for MF1002, implying that these three enzyme activities may be correlation with the high sugar tolerance of MF11a, and could be used as the indicator for further improvement of the strain.