基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2013年
5期
575-580
,共6页
王世银%张伟%沈敏%李欢%梁耀伟%杨井泉%高磊%甘尚权%王新华
王世銀%張偉%瀋敏%李歡%樑耀偉%楊井泉%高磊%甘尚權%王新華
왕세은%장위%침민%리환%량요위%양정천%고뢰%감상권%왕신화
脂臀性状%多态性%等位基因%基因型%分子标记
脂臀性狀%多態性%等位基因%基因型%分子標記
지둔성상%다태성%등위기인%기인형%분자표기
Fat-rump trait%Polymorphism%Allele gene%Genotype%Molecular marker
尾脂沉积能力在脂尾型绵羊和瘦尾型绵羊间存在很大的差异，但其沉积脂肪的遗传特性与分子机制仍不明晰。为此，本研究选择尾型极端差异的阿勒泰羊(巨型脂臀)、湖羊(短脂尾)和黑萨福克羊(长瘦尾)，采用PCR-RFLP方法检测绵羊X染色体59194976位点多态性，分析其与尾(臀)脂沉积能力的相关性。结果表明：在阿勒泰羊群体中G等位基因属优势等位基因，其两等位基因比值(G/A)分别是湖羊和黑萨福克羊的6倍和68倍，且G等位基因在阿勒泰羊中趋于纯合。卡方检验结果表明，阿勒泰羊在该位点均处于Hardy-Weinberg平衡状态(p>0.05)，而湖羊和黑萨福克羊在该位点均处于Hardy-Weinberg极不平衡状态(p<0.01)。以上结果提示，绵羊X染色体59194976位点SNP可作为理想的分子标记应用于高、低脂绵羊品种选育。
尾脂沉積能力在脂尾型綿羊和瘦尾型綿羊間存在很大的差異，但其沉積脂肪的遺傳特性與分子機製仍不明晰。為此，本研究選擇尾型極耑差異的阿勒泰羊(巨型脂臀)、湖羊(短脂尾)和黑薩福剋羊(長瘦尾)，採用PCR-RFLP方法檢測綿羊X染色體59194976位點多態性，分析其與尾(臀)脂沉積能力的相關性。結果錶明：在阿勒泰羊群體中G等位基因屬優勢等位基因，其兩等位基因比值(G/A)分彆是湖羊和黑薩福剋羊的6倍和68倍，且G等位基因在阿勒泰羊中趨于純閤。卡方檢驗結果錶明，阿勒泰羊在該位點均處于Hardy-Weinberg平衡狀態(p>0.05)，而湖羊和黑薩福剋羊在該位點均處于Hardy-Weinberg極不平衡狀態(p<0.01)。以上結果提示，綿羊X染色體59194976位點SNP可作為理想的分子標記應用于高、低脂綿羊品種選育。
미지침적능력재지미형면양화수미형면양간존재흔대적차이，단기침적지방적유전특성여분자궤제잉불명석。위차，본연구선택미형겁단차이적아륵태양(거형지둔)、호양(단지미)화흑살복극양(장수미)，채용PCR-RFLP방법검측면양X염색체59194976위점다태성，분석기여미(둔)지침적능력적상관성。결과표명：재아륵태양군체중G등위기인속우세등위기인，기량등위기인비치(G/A)분별시호양화흑살복극양적6배화68배，차G등위기인재아륵태양중추우순합。잡방검험결과표명，아륵태양재해위점균처우Hardy-Weinberg평형상태(p>0.05)，이호양화흑살복극양재해위점균처우Hardy-Weinberg겁불평형상태(p<0.01)。이상결과제시，면양X염색체59194976위점SNP가작위이상적분자표기응용우고、저지면양품충선육。
The ability of fat deposition has a great difference between the fat-tail and thin-tail sheep, but the heredity and molecule mechanism of fat accumulation is poorly understood. In recent documents, the single nucleotide polymorphsim (SNP) locus at the 59194976 nucleotide of X chromosome was reported as a moleculer marker of fat accumulation. In this study, using PCR-RFLP method, the Genotypes and allele frequencies distribu-tions was analyzed in three sheep breeds of Altay, Hu and Suffolk, which there are extremely difference on tail type. The result showed that G allele gene of 59194976 locus is preponderant allele gene in the Altay which is fat-tail or fat-rump sheep breed, the ratio of two allele gene (G/A) is 6 and 68 times compared with Hu and Suffolk sheep, and the G allele gene has a homozygosity trend in Altay sheep. The result of chi-square analysis suggested that the allele frequencies and genotype frequencies were in Hardy-Weinberg equilibrium (p>0.05) in Altay sheep, but were not in Hardy-Weinberg equilibrium (p<0.01) in Hu and Suffolk sheep. This study demonstrated that the SNP locus of 59194976th on X chromosome was a perfect molecular marker of sheep breeding in high or low fat.
