世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
6期
1338-1342
,共5页
小建中汤%拆方%芍药苷%含量测定%高效液相色谱法
小建中湯%拆方%芍藥苷%含量測定%高效液相色譜法
소건중탕%탁방%작약감%함량측정%고효액상색보법
Xiao -Jian -Zhong decoction%disassembled prescription%paeoniflorin%content determination%high performance liquid chromatography
目的：研究小建中汤及其拆方对芍药苷含量的影响。方法：采用Diamonsil RP-C18色谱柱（250 mm×4.6 mm，5μm），以乙腈-水为流动相，流速1.0 mL·min-1，检测波长230 nm。结果：小建中汤拆方前后芍药苷的含量有明显变化，其中去甘草组、去桂枝组、去饴糖组与全方组比较均有显著性差异（P<0.05）；去生姜组、去大枣组与全方组比较无显著性差异（P>0.05）。结论：甘草和桂枝有助于白芍中芍药苷的溶出；大枣、生姜对白芍中芍药苷的溶出影响不大；饴糖可能会以小团块形式包裹芍药苷，使实验中芍药苷的萃取效率降低。
目的：研究小建中湯及其拆方對芍藥苷含量的影響。方法：採用Diamonsil RP-C18色譜柱（250 mm×4.6 mm，5μm），以乙腈-水為流動相，流速1.0 mL·min-1，檢測波長230 nm。結果：小建中湯拆方前後芍藥苷的含量有明顯變化，其中去甘草組、去桂枝組、去飴糖組與全方組比較均有顯著性差異（P<0.05）；去生薑組、去大棘組與全方組比較無顯著性差異（P>0.05）。結論：甘草和桂枝有助于白芍中芍藥苷的溶齣；大棘、生薑對白芍中芍藥苷的溶齣影響不大；飴糖可能會以小糰塊形式包裹芍藥苷，使實驗中芍藥苷的萃取效率降低。
목적：연구소건중탕급기탁방대작약감함량적영향。방법：채용Diamonsil RP-C18색보주（250 mm×4.6 mm，5μm），이을정-수위류동상，류속1.0 mL·min-1，검측파장230 nm。결과：소건중탕탁방전후작약감적함량유명현변화，기중거감초조、거계지조、거이당조여전방조비교균유현저성차이（P<0.05）；거생강조、거대조조여전방조비교무현저성차이（P>0.05）。결론：감초화계지유조우백작중작약감적용출；대조、생강대백작중작약감적용출영향불대；이당가능회이소단괴형식포과작약감，사실험중작약감적췌취효솔강저。
This study was aimed to investigate the effect of Xiao-Jian-Zhong decoction and its disassembled prescriptions on the content determination of paeoniflorin . A gradient HPLC system was used in the quantitative analysis of paeoniflorin on Diamonsil RP-C18 column ( 250 mm × 4 . 6 mm , 5 μm ) with the mixed solvents of acetonitrile and water . The flow rate was 1 . 0 mL?min-1 and the detection wavelength was 230 nm . The results showed that the content of paeoniflorin had great changes comparing with X iao-Jian-Zhong decoction with its different disassembled prescriptions . The analysis of variance showed that the difference among groups was sig-nificant (P < 0.05). SNKq analysis showed that there was significant difference between groups 6 and 1, 6 and 2 , 6 and 5 ( P < 0 . 05 ) . But no significant difference was existed among group 6 , 3 and 4 ( P > 0 . 05 ) . It was concluded that liquorice and cassia twigs were helpful to the dissolution of paeoniflorin in the decoction . But jujube and ginger had few effects . The content of paeoniflorin in group 5 which does not include maltose was the highest among all groups . It may be due to the fact that paeoniflorin was wrapped in the maltose of X iao-Jian-Zhong decoction .
