CAJ | 학술논문

本文以道地药材黄连的研究为例，对其生长环境进行分析，运用ISSR等方法考察其遗传型，采用HPLC、1H-NMR及NIR等方法对化学型进行分析，并从抗内毒素、抗微生物的生物热力学，以及治疗糖尿病相关的胰岛素抵抗生物效应等角度考察其药效型，综合分析其相关性，初步建立了基于黄连生态型、基因型、化学型和药效型的道地药材品质评价方法，同时建立了结合道地性的黄连规范化质量标准，为构建体现中医药特色的黄连品质评价方法提供了科学依据。
본문이도지약재황련적연구위례，대기생장배경진행분석，운용ISSR등방법고찰기유전형，채용HPLC、1H-NMR급NIR등방법대화학형진행분석，병종항내독소、항미생물적생물열역학，이급치료당뇨병상관적이도소저항생물효응등각도고찰기약효형，종합분석기상관성，초보건립료기우황련생태형、기인형、화학형화약효형적도지약재품질평개방법，동시건립료결합도지성적황련규범화질량표준，위구건체현중의약특색적황련품질평개방법제공료과학의거。
This study took geo-authentic medicinal material Rhizoma Coptidis as an example and analyzed soil characteristics of the growing environment of Rhizoma Coptidis . The methods of ISSR, HPLC, 1H-NMR and NIR were respectively used for exploring its genotype and investigating on its chemitype . In the respects of an-ti-endotoxin, anti-bacterial activities and insulin resistance that was related to the treatment of diabetes, the pharmacodynamic type was studied and the relationship was comprehensively analyzed . The quality evaluation of geo-authentic medicinal materials was preliminarily established based on ecotype , genotype chemitype and phar-macodynamic type of Rhizoma Coptidis . Simultaneously, quality standards of Rhizoma Coptidis were set up in combination with genuine character , which provided the scientific basis for establishing Chinese medicine char-acteristic methods of quality evaluation of Rhizoma Coptidis .