CAJ | 학술논문

目的：研究证实男性Ⅱ型糖尿病患者血清睾酮水平下降，但其机制尚不清楚。本研究旨在探讨Ⅱ型糖尿病对模型小鼠睾丸间质细胞睾酮分泌相关因子的影响。方法采用雄性3月龄obob小鼠，检测其血清睾酮水平，应用Westren-blot技术检测模型小鼠睾丸间质细胞类固醇激素合成急性调节蛋白（StAR）、胆固醇侧链裂解酶（P450scc）、3β-羟类固醇脱氢酶（3βHSD）的表达水平。结果雄性obob小鼠血清睾酮水平明显低于对照组；obob小鼠睾丸间质细胞StAR 、P450scc和3βHSD表达水平明显下降。结论下调睾丸间质细胞StAR 、P450scc和3βHSD表达水平可能是Ⅱ型糖尿病导致血清睾酮水平下降的原因。
목적：연구증실남성Ⅱ형당뇨병환자혈청고동수평하강，단기궤제상불청초。본연구지재탐토Ⅱ형당뇨병대모형소서고환간질세포고동분비상관인자적영향。방법채용웅성3월령obob소서，검측기혈청고동수평，응용Westren-blot기술검측모형소서고환간질세포류고순격소합성급성조절단백（StAR）、담고순측련렬해매（P450scc）、3β-간류고순탈경매（3βHSD）적표체수평。결과웅성obob소서혈청고동수평명현저우대조조；obob소서고환간질세포StAR 、P450scc화3βHSD표체수평명현하강。결론하조고환간질세포StAR 、P450scc화3βHSD표체수평가능시Ⅱ형당뇨병도치혈청고동수평하강적원인。
Objective To study the effects of Diabetes MellitusⅡon the secretion of testosterone in Leydig cells of the model mouse. Methods Levels of serum testosterone in the model mouse and the control were detected. The expression of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc) and 3 beta hydroxy steroid dehydrogenase (3βHSD) in Leydig cells of the model mouse were measured by western blot, respectively. Results The level of serum testosterone in male obob mouse was significantly lower than that in the control;The expression of StAR, P450scc and 3βHSD in Leydig cells of obob mouse remarkably decreased. Conclusion What diabetes mellitusⅡcauses the decline of the level of serum testosterone might be associated with low expression of StAR、P450scc and 3βHSD.