CAJ | 학술논문

为加强紫薇分子遗传学研究，以紫薇叶片DNA为模板，采用4因素（模板浓度、dNTP、引物浓度、酶浓度）3水平的L9（34）正交设计，优化紫薇的SSR-PCR反应体系，并对24对紫薇SSR引物进行了筛选。结果表明：紫薇10μLSSR-PCR的最优反应体系为：Taq酶0．2 U、Mg2＋2．5 mmol·L-1、dNTP 0．15 mmol·L-1、F-primer 0．25 mmol·L-1、R-primer 0．25 mmol·L-1、DNA 60 ng ，ddH2 O补至10μL ；24对EST-SSR引物筛选出可扩增出条带的引物23对，其中16对具有多态性，共扩增出清晰条带68条，差异条带39条。
위가강자미분자유전학연구，이자미협편DNA위모판，채용4인소（모판농도、dNTP、인물농도、매농도）3수평적L9（34）정교설계，우화자미적SSR-PCR반응체계，병대24대자미SSR인물진행료사선。결과표명：자미10μLSSR-PCR적최우반응체계위：Taq매0．2 U、Mg2＋2．5 mmol·L-1、dNTP 0．15 mmol·L-1、F-primer 0．25 mmol·L-1、R-primer 0．25 mmol·L-1、DNA 60 ng ，ddH2 O보지10μL ；24대EST-SSR인물사선출가확증출조대적인물23대，기중16대구유다태성，공확증출청석조대68조，차이조대39조。
In order to further research the molecular markers of L agerstroemia indica L .,taking DNA of L a-gerstroemia indica L .as template ,several important parameters influencing SSR-PCR amplification were stud-ied with an orthogonal design by four factors and three levels ,as to establish the optimum SSR-PCR reaction system ,and the primers were screened from 24 pairs of SSR primers .The results showed that the optimal SSR-PCR reaction system was established as a total volume of 10 μL including Taq DNA polymerase 0 .2 U ,Mg2+2 .5 mmol·L-1 ,dNTPs 0 .15 mmol·L-1 ,0 .25 mmol·L-1 of each primer and template DNA 60 ng ,deionized water to 10 μL .23 pairs of available primers were screened from 24 pairs of EST-SSR primers ,there were 16 pairs of polymorphism primers amplified out 68 clear bands ,39 bands with differences .