CAJ | 학술논문

目的：研究苯妥英钠（PHT）对内毒素作用下人牙周膜干细胞（hPDLSCs）增殖的影响。方法本研究于2009年8月至2012年6月在滨州医学院中心实验室和西安交通大学医学院中心实验室完成。采用四唑盐（MTT）比色方法，测定体外培养的hPDLSCs分别在20μg/mL PHT+100 ng/mL脂多糖（LPS）、5μg/mL PHT+100 ng/mL LPS、20μg/mL PHT和5μg/mL PHT作用下的增殖活性。结果20μg/mL PHT和5μg/mL PHT均能显著促进hPDLSCs的增殖，且20μg/mL PHT促进增殖的能力显著强于5μg/mL PHT；加入100 ng/mL LPS对20μg/mL和5μg/mL PHT促进增殖能力均无明显影响。结论低浓度PHT能促进hPDLSCs的增殖，少量内毒素的存在并不影响PHT促进hPDLCs增殖的生物学作用。
목적：연구분타영납（PHT）대내독소작용하인아주막간세포（hPDLSCs）증식적영향。방법본연구우2009년8월지2012년6월재빈주의학원중심실험실화서안교통대학의학원중심실험실완성。채용사서염（MTT）비색방법，측정체외배양적hPDLSCs분별재20μg/mL PHT+100 ng/mL지다당（LPS）、5μg/mL PHT+100 ng/mL LPS、20μg/mL PHT화5μg/mL PHT작용하적증식활성。결과20μg/mL PHT화5μg/mL PHT균능현저촉진hPDLSCs적증식，차20μg/mL PHT촉진증식적능력현저강우5μg/mL PHT；가입100 ng/mL LPS대20μg/mL화5μg/mL PHT촉진증식능력균무명현영향。결론저농도PHT능촉진hPDLSCs적증식，소량내독소적존재병불영향PHT촉진hPDLCs증식적생물학작용。
Objective To evaluate the effects of phenytoin(PHT)on proliferation of human periodontal ligament stem cells(hPDLSCs)under existing lipopolysaccharide(LPS). Methods MTT method was used to observe the proliferation of hPDLSCs cultured in vitro under the action of 20μg/mL PHT+100 ng/mL LPS,5μg/mL PHT+100 ng/mL LPS,20μg/mL PHT and 5μg/mL PHT,respectively. Results Both 20μg/mL and 5μg/mL PHT could enhance the proliferation of hPDLSCs,and furthermore the effect of 20μg/mL PHT was stronger. The enhancing effects of 20μg/mL and 5μg/mL PHT on the proliferation of hPDLSCs remained no change when 100 ng/mL LPS was added. Conclusion PHT at low concentration can enhance the proliferation of hPDLSCs;a small amount of LPS does not affect biological effect of PHT in enhancing the proliferation of hPDLSCs.