CAJ | 학술논문

目的：探讨三七总皂苷(PNS)对大鼠脊髓半横断损伤后继发性损害的神经保护作用及相关机制。方法55只成年Sprague-Dawley大鼠随机分成3组：假手术组(n=5)、脊髓损伤组(n=25)和PNS组(n=25)。造模成功后1 d、3 d、7 d、14 d和21 d应用BBB评分标准对大鼠后肢运动功能进行行为学评分，相应时间点取材后对大鼠脊髓组织进行病理形态学观察和免疫组化分析。结果 PNS干预后3 d、7 d、14 d和21 d时PNS组大鼠运动功能恢复优于脊髓损伤组(P<0.05)。7 d、14 d和21 d时PNS组尼氏染色示神经元坏死减少，形态完整，且胞浆型磷脂酶A2(cPLA2)阳性细胞数减少，神经元形态完整，cPLA2表达增强被抑制。结论PNS可通过抑制cPLA2表达在脊髓损伤中发挥神经保护作用，促进运动功能恢复。
목적：탐토삼칠총조감(PNS)대대서척수반횡단손상후계발성손해적신경보호작용급상관궤제。방법55지성년Sprague-Dawley대서수궤분성3조：가수술조(n=5)、척수손상조(n=25)화PNS조(n=25)。조모성공후1 d、3 d、7 d、14 d화21 d응용BBB평분표준대대서후지운동공능진행행위학평분，상응시간점취재후대대서척수조직진행병리형태학관찰화면역조화분석。결과 PNS간예후3 d、7 d、14 d화21 d시PNS조대서운동공능회복우우척수손상조(P<0.05)。7 d、14 d화21 d시PNS조니씨염색시신경원배사감소，형태완정，차포장형린지매A2(cPLA2)양성세포수감소，신경원형태완정，cPLA2표체증강피억제。결론PNS가통과억제cPLA2표체재척수손상중발휘신경보호작용，촉진운동공능회복。
Objective To investigate the protective effect and cytosolic phospholipase A2 (cPLA2) associated mechanism of panax no-toginseng saponins (PNS) in spinal cord hemisection injured rats. Methods 55 adult Sprague-Dawley rats were randomly divided into three groups:sham group (n=5), spinal cord injury group (n=25) and PNS group (n=25). The rats were evaluated with BBB score, pathology and immunohistochemistry 1 d, 3 d, 7 d, 14 d and 21 d after intervention. Results Compared to the spinal cord injury group, motor recovery was significantly better in PNS group 3 d, 7 d, 14 d and 21 d after intervention (P<0.05). Nissl staining showed less neuron necrosis and more in-tegrated neural cells in morphology in PNS group 7 d, 14 d and 21 d after intervention. Cytosolic phospholipase A2 (cPLA2) expression was inhabited, and less number of positive cells were found in PNS group 7 d, 14 d and 21 d after intervention. Conclusion PNS can inhibit the expression of cPLA2 after spinal cord injury, which may be one of the mechanisms of its effect on promoting motor recovery.