CAJ | 학술논문

根据基因库中沙门氏菌、空肠弯曲杆菌、单核细胞增生李斯特菌和大肠杆菌O157：H7的invA、MapA、hlyA和O gene cluster基因分别设计了4对引物，通过对反应条件的优化，建立了同时检测4种病原菌的多重PCR方法。结果表明，该多重PCR方法可扩增出四条特异性条带，并且任意两条产物片段长度相差大于20％。多重PCR反应体系检测四种病原菌混合模板最低含量为100 CFU。该多重PCR检测方法具有快速、准确和特异性强的优点，可用于快速检测食品中的病原菌。
근거기인고중사문씨균、공장만곡간균、단핵세포증생리사특균화대장간균O157：H7적invA、MapA、hlyA화O gene cluster기인분별설계료4대인물，통과대반응조건적우화，건립료동시검측4충병원균적다중PCR방법。결과표명，해다중PCR방법가확증출사조특이성조대，병차임의량조산물편단장도상차대우20％。다중PCR반응체계검측사충병원균혼합모판최저함량위100 CFU。해다중PCR검측방법구유쾌속、준학화특이성강적우점，가용우쾌속검측식품중적병원균。
Four pairs of primers were designed according to the sequences of invA gene of Salmonella,MapA gene of Campylobacter jejuni,hlyA gene of Listeria monocytohenes and O gene cluster of Escherichia coli O157:H7. After op-timization of conditions,a multiplex-PCR assay was established for rapid detection of the 4 common pathogens in food. Results showed that the multiplex PCR assay could amplify four speciifc bands and more than 20%difference in length existed between any two of them,and the sensitivity of the PCR system was at least 100 CFU mixed templates of the 4 pathogens. The multiplex-PCR assay was rapid,accurate and speciifc in operation and could be used in food inspection.