CAJ | 학술논문

同时采用间接ELISA、虎红平板凝集试验和试管凝集试验三种血清学方法调查川西北牦牛布鲁氏菌病血清流行情况。对1070份采自川西北阿坝州8个草地县的牦牛血清样品经三种血清学方法检测，间接ELISA检测阳性率为25.4%（272/1070），虎红平板凝集试验检测阳性率为12.7%（136/1070），而试管凝集试验检测阳性率为9.1%（97/1070）。8个县均发现阳性布鲁氏菌病血清。与间接ELISA相比，虎红平板凝集试验的相对敏感性和特异性分别为50%和100%，而试管凝集试验的相对敏感性和特异性分别为35.7%和100%。布鲁氏菌病在川西北地区广泛存在，血清学方法是检测和监测布鲁氏菌病最常用和最简便的方法，其中间接ELISA方法最为敏感。
동시채용간접ELISA、호홍평판응집시험화시관응집시험삼충혈청학방법조사천서북모우포로씨균병혈청류행정황。대1070빈채자천서북아패주8개초지현적모우혈청양품경삼충혈청학방법검측，간접ELISA검측양성솔위25.4%（272/1070），호홍평판응집시험검측양성솔위12.7%（136/1070），이시관응집시험검측양성솔위9.1%（97/1070）。8개현균발현양성포로씨균병혈청。여간접ELISA상비，호홍평판응집시험적상대민감성화특이성분별위50%화100%，이시관응집시험적상대민감성화특이성분별위35.7%화100%。포로씨균병재천서북지구엄범존재，혈청학방법시검측화감측포로씨균병최상용화최간편적방법，기중간접ELISA방법최위민감。
The indirect ELISA(iELISA),the Rose bengal plate test(RBPT)and standard tube agglutination test(SAT), were used to investigate the serological prevalence of bovine brucellosis in yak in regions ordering northwestern Sichuan province of China. A total of 1070 serum samples collected from 8 counties in the grassland area in northwest Sichuan province of China,were examined to investigate the brucellosis sero-prevalence by the three methods. Serological evi-dence indicated that the apparent prevalence of brucellosis in yaks was 25.4%(272/1070) detected by iELISA,12.7%(136/1070)by RBPT,and 9.1%(97/1070) by STAT,suggesting that yak brucellosis existed in all the 8 counties under in the study. The relative sensitivity and speciifcity of RBPT were 50.0%and 100%,those of STAT were 35.7%and 100%respectively,compared with iELISA. The results shown that iELISA was the most sensitive method.