畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2013年
10期
13-16,17
,共5页
甘氨酰谷氨酰胺二肽%脂肪代谢%ACC%PPARα
甘氨酰穀氨酰胺二肽%脂肪代謝%ACC%PPARα
감안선곡안선알이태%지방대사%ACC%PPARα
glycyl-glutamine dipeptide%lipid metabolism%ACC%PPARα
选择360只1日龄优质黄羽肉鸡，随机分为4组，分别饮用凉开水配制的浓度为0、0.01、0.05和0.10 mg/L的甘氨酰谷氨酰胺（Gly-Gln）二肽溶液，连续饮用41 d。用甘油三酯试剂盒测定肝脏总甘油三酯的含量。采用相对定量RT-PCR的方法来检测ACC（acetyl-CoA carboxylase）和PPARα（peroxisome proliferator-activated receptorsα）mRNA的表达水平。试验结果表明：①Gly-Gln二肽能够呈现剂量依赖性的降低肝脏ACC mRNA的表达水平，0.10 mg/L Gly-Gln二肽处理组较对照组差异显著（P<0.05）。②Gly-Gln二肽能够显著上调PPARα基因mRNA的表达水平，其中0.05 mg/L Gly-Gln二肽处理组较对照组差异显著（P<0.05）。③Gly-Gln二肽具有提高肝脏甘油三酯含量的趋势，但差异不显著（P>0.05）。以上结果提示，Gly-Gln二肽能够通过降低肝脏甘油三酯的合成，促进代谢，从而减少脂肪沉积水平。
選擇360隻1日齡優質黃羽肉鷄，隨機分為4組，分彆飲用涼開水配製的濃度為0、0.01、0.05和0.10 mg/L的甘氨酰穀氨酰胺（Gly-Gln）二肽溶液，連續飲用41 d。用甘油三酯試劑盒測定肝髒總甘油三酯的含量。採用相對定量RT-PCR的方法來檢測ACC（acetyl-CoA carboxylase）和PPARα（peroxisome proliferator-activated receptorsα）mRNA的錶達水平。試驗結果錶明：①Gly-Gln二肽能夠呈現劑量依賴性的降低肝髒ACC mRNA的錶達水平，0.10 mg/L Gly-Gln二肽處理組較對照組差異顯著（P<0.05）。②Gly-Gln二肽能夠顯著上調PPARα基因mRNA的錶達水平，其中0.05 mg/L Gly-Gln二肽處理組較對照組差異顯著（P<0.05）。③Gly-Gln二肽具有提高肝髒甘油三酯含量的趨勢，但差異不顯著（P>0.05）。以上結果提示，Gly-Gln二肽能夠通過降低肝髒甘油三酯的閤成，促進代謝，從而減少脂肪沉積水平。
선택360지1일령우질황우육계，수궤분위4조，분별음용량개수배제적농도위0、0.01、0.05화0.10 mg/L적감안선곡안선알（Gly-Gln）이태용액，련속음용41 d。용감유삼지시제합측정간장총감유삼지적함량。채용상대정량RT-PCR적방법래검측ACC（acetyl-CoA carboxylase）화PPARα（peroxisome proliferator-activated receptorsα）mRNA적표체수평。시험결과표명：①Gly-Gln이태능구정현제량의뢰성적강저간장ACC mRNA적표체수평，0.10 mg/L Gly-Gln이태처리조교대조조차이현저（P<0.05）。②Gly-Gln이태능구현저상조PPARα기인mRNA적표체수평，기중0.05 mg/L Gly-Gln이태처리조교대조조차이현저（P<0.05）。③Gly-Gln이태구유제고간장감유삼지함량적추세，단차이불현저（P>0.05）。이상결과제시，Gly-Gln이태능구통과강저간장감유삼지적합성，촉진대사，종이감소지방침적수평。
360 one-day old yellow-feathered broilers were randomly divided into four groups to drink with 0, 0.01, 0.05 and 0.10 mg/L glycyl-glutamine dipeptide (made with cool boiled water) respectively for 41 days. The content of TG in the liver was determined by commercial TG kit. By using semi-quantitative RT-PCR,mRNA expression level of ACC and PPAR α were detected. The results showed that Gly-Gln dipetide dose-dependently decreased the expression level of ACC mRNA in the liver and that in 0.10 mg/L Gly-Gln dipetide group had significant difference with control group (P<0.05). Gly-Gln dipetide could significantly increased the expression level of PPAR α mRNA and that in 0.05 mg/L Gly-Gln dipetide group had significant difference with control group(P<0.05). Gly-Gln dipetide had the trend of increasing the TG content in the liver, but the difference was not significant(P>0.05). Therefore Gly-Gln dipetide could decrease the synthesis of TG in the liver and promote metabolism, so as to reduce body fat deposition.
