CAJ | 학술논문

对离子束诱变所产生的共轭亚油酸（CLA）下调的植物乳杆菌p8中，转录因子rpoD基因序列的变化进行分析。通过PCR扩增获得野生型和几株突变型菌株的rpoD基因，构建到pMD-19T载体上，转化大肠杆菌DH5α，经菌落PCR和测序分析筛选与鉴定阳性克隆子，并对野生型和突变型菌株的rpoD基因序列预测编码的氨基酸进行差异比较。结果表明，突变菌株的rpoD基因并未发生突变， CLA产量下调并不是由转录因子rpoD突变所引起。
대리자속유변소산생적공액아유산（CLA）하조적식물유간균p8중，전록인자rpoD기인서렬적변화진행분석。통과PCR확증획득야생형화궤주돌변형균주적rpoD기인，구건도pMD-19T재체상，전화대장간균DH5α，경균락PCR화측서분석사선여감정양성극륭자，병대야생형화돌변형균주적rpoD기인서렬예측편마적안기산진행차이비교。결과표명，돌변균주적rpoD기인병미발생돌변， CLA산량하조병불시유전록인자rpoD돌변소인기。
The change of gene sequences of transcription factor-rpoD which comes from mutated Lactobacillus plantarum p8 by the ion beam with decreasing conjugated linoleic acid (CLA) is analyzed. The rpoD's gene of wild-type and mutant Lactobacillus plantarum p8 are obtained by PCR, recombined into vector-pMD-19T, transformed into Ecoli. DH5α, and identified by clone PCR and sequencing compared the amino acids sequencing. The result indicates that there is nothing mutations in the rpoD's gene sequences and decrease of CLA is not caused by transcription factor-rpoD mutations.