CAJ | 학술논문

为研制粘帚霉生防制剂，以粉红粘帚霉67-1为材料，对粉红粘帚霉67-1厚垣孢子的生长过程、孢子结构、贮存介质和制剂含水量以及厚垣孢子和分生孢子的货架期比较进行了研究。结果表明：在特定的培养基中，粉红粘帚霉67-1在培养24 h后产生厚垣孢子，厚垣孢子形成于菌丝顶端或中部，并可以脱落游离到培养基中。电镜下可以看到圆形或椭圆形厚垣孢子以及加厚的细胞壁。相同贮存条件下，厚垣孢子的货架期比分生孢子长。低温条件下，10％含水量的贮存介质更利于厚垣孢子的贮存。
위연제점추매생방제제，이분홍점추매67-1위재료，대분홍점추매67-1후원포자적생장과정、포자결구、저존개질화제제함수량이급후원포자화분생포자적화가기비교진행료연구。결과표명：재특정적배양기중，분홍점추매67-1재배양24 h후산생후원포자，후원포자형성우균사정단혹중부，병가이탈락유리도배양기중。전경하가이간도원형혹타원형후원포자이급가후적세포벽。상동저존조건하，후원포자적화가기비분생포자장。저온조건하，10％함수량적저존개질경리우후원포자적저존。
In order to develop Gliocladium biocontrol preparation ,taking Clonostachys rosea 67-1 chlamydospores as test materials ,the growth process ,morphology ,shelf life ,storage media ,water content and comparation between chla-mydospores and conidia were studied .The results showed that chlamydospores produced in specific medium after cul-turing 24 hours ,chlamydospores formed in the centre of hyphae or at the top ,and it could fall off into the medium . Circular or ellipse thick wall structure and incrassate cell wall could be seen under the electron microscope .In the same storage conditions ,chlamydospores had longer shelf life than condia .Under low temperature ,the storage medium of 10% water content could extend shelf life of chlamydospores of Clonostachys rosea 67-1 .