光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2013年
11期
3055-3060
,共6页
荧光光谱法%银杏%黄酮糖苷
熒光光譜法%銀杏%黃酮糖苷
형광광보법%은행%황동당감
Fluorescence spectrophotometry%Ginkgo%Flavonoids glycosides
银杏黄酮糖苷因具有多种生物活性而被制药、食品、日用品等诸多领域广泛应用。针对目前银杏黄酮糖苷检测操作繁琐、成本高、耗时长的不足，建立了一种高精确、低成本的快速测定方法。根据黄酮类化合物能与A l3＋反应形成荧光螯合物的原理，以芦丁为标样探索测试条件，结果表明在激发波长λex ＝400 nm ，发射波长λem ＝520 nm ，pH为3.6的Al（NO3）3-（HAc-NaAc）反应体系中反应1500 s ，螯合物荧光强度趋于稳定且达到最大值。求得芦丁浓度与荧光值的线性回归方程为 y＝29.92 x＋36.49（R2＝0.986），线性范围为1.8×10-6～3.2×10-5 mol·L -1。用这种方法检测了银杏悬浮培养细胞中黄酮糖苷的含量，并进行加标回收实验，平均回收率为101.3％。该方法灵敏度高，重现性好，操作简单，具有良好的应用前景。
銀杏黃酮糖苷因具有多種生物活性而被製藥、食品、日用品等諸多領域廣汎應用。針對目前銀杏黃酮糖苷檢測操作繁瑣、成本高、耗時長的不足，建立瞭一種高精確、低成本的快速測定方法。根據黃酮類化閤物能與A l3＋反應形成熒光螯閤物的原理，以蘆丁為標樣探索測試條件，結果錶明在激髮波長λex ＝400 nm ，髮射波長λem ＝520 nm ，pH為3.6的Al（NO3）3-（HAc-NaAc）反應體繫中反應1500 s ，螯閤物熒光彊度趨于穩定且達到最大值。求得蘆丁濃度與熒光值的線性迴歸方程為 y＝29.92 x＋36.49（R2＝0.986），線性範圍為1.8×10-6～3.2×10-5 mol·L -1。用這種方法檢測瞭銀杏懸浮培養細胞中黃酮糖苷的含量，併進行加標迴收實驗，平均迴收率為101.3％。該方法靈敏度高，重現性好，操作簡單，具有良好的應用前景。
은행황동당감인구유다충생물활성이피제약、식품、일용품등제다영역엄범응용。침대목전은행황동당감검측조작번쇄、성본고、모시장적불족，건립료일충고정학、저성본적쾌속측정방법。근거황동류화합물능여A l3＋반응형성형광오합물적원리，이호정위표양탐색측시조건，결과표명재격발파장λex ＝400 nm ，발사파장λem ＝520 nm ，pH위3.6적Al（NO3）3-（HAc-NaAc）반응체계중반응1500 s ，오합물형광강도추우은정차체도최대치。구득호정농도여형광치적선성회귀방정위 y＝29.92 x＋36.49（R2＝0.986），선성범위위1.8×10-6～3.2×10-5 mol·L -1。용저충방법검측료은행현부배양세포중황동당감적함량，병진행가표회수실험，평균회수솔위101.3％。해방법령민도고，중현성호，조작간단，구유량호적응용전경。
Flavonoids glycosides from ginkgo are widely applied in pharmaceutical ,food ,daily necessities and other areas due to their variety of biological activities .To overcome the shortcoming of current methods of time wasting ,complex operation and expensive costs ,an accuracy ,low costs and fast determination method was established for flavonoids glycosides from ginkgo . According to the principle that flavonoids can form fluorescent chelate with Al 3+ ,the detection condition was explored with rutin as standard .The results showed that the fluorimetric intensy of chelate of rutin with Al 3+ would be stabilized and achieve maxi-mum with λex =400 nm and λem =520 nm ,in Al(NO3 )3-(HAc-NaAc) reaction system for 1 500 s with pH 3.6 .The linear re-gression equation y=29.92x+36.49 (R2 =0.986) was deduced with the concentration of rutin and fluorescence intensy ,and the linear range 1.8 × 10-6 ~3.2 × 10-5 mol · L -1 .Flavonoids glycosides of cell suspension cultures from ginkgo biloba was detec-ted by this method .The recovery experiments were also carried out with the average recovery rate of 101.3% .The advantages of high sensitivity ,reproducibility ,simple operation ,and low costs were showed ,indicating its good prospects .
