CAJ | 학술논문

为探索微生物胞外物在微生物对重金属的抗性和去除过程中的作用，比较分析了对重金属镉具有不同抗性和去除率的菌株SCSE425-7和SCSE709-6胞外物的产生情况和菌株的红外光谱谱图。结果表明，在含镉条件培养时，菌株SCSE425-7表现了较高的镉抗性，分泌了相对较多的可溶性胞外多糖；而菌株SCSE709-6镉抗性较SCSE425-7低，但镉的生物去除性能较好，分泌了更多不可溶胞外聚合物。这说明，在重金属毒性胁迫下，微生物分泌的可溶性胞外多糖可能有助于提高微生物对重金属的抗性，而不可溶性胞外聚合物有助于重金属的微生物吸附。菌株红外光谱分析结果表明不可溶胞外聚合物上的酰胺基和羧基是其吸附镉离子的主要官能团。
위탐색미생물포외물재미생물대중금속적항성화거제과정중적작용，비교분석료대중금속력구유불동항성화거제솔적균주SCSE425-7화SCSE709-6포외물적산생정황화균주적홍외광보보도。결과표명，재함력조건배양시，균주SCSE425-7표현료교고적력항성，분비료상대교다적가용성포외다당；이균주SCSE709-6력항성교SCSE425-7저，단력적생물거제성능교호，분비료경다불가용포외취합물。저설명，재중금속독성협박하，미생물분비적가용성포외다당가능유조우제고미생물대중금속적항성，이불가용성포외취합물유조우중금속적미생물흡부。균주홍외광보분석결과표명불가용포외취합물상적선알기화최기시기흡부력리자적주요관능단。
In order to explore the effect of extracelluar polymeric substances (EPS) on resistance and removal of heavy metals , the production of EPS ,secreted by cadmium-resistant strain (SCSE425-7) and cadmium-removal strain (SCSE709-6) was inves-tigated combined with Fourier transform infrared spectroscopy (FTIR) .The results showed that the high resistance to cadmium of strain SCSE425-7 was related to the high production of soluble EPS ,whereas SCSE709-6 secreted more insoluble EPS resul-ting in better cadmium removal performance .It was indicated that soluble extracellular carbohydrates may help the bacteria to enhance resistance to Cd2+ ,and insoluble EPS could contribute to Cd2+ removal effectively .The FTIR spectra showed that the peaks of amide and carboxyl were main functional groups for Cd2+ adsorption .