CAJ | 학술논문

应用傅里叶变换红外光谱技术监测乳腺癌细胞株 MCF-7在化疗药物5氟尿嘧啶（5-FU ）干预过程中相应的红外光谱变化，验证其与药物作用的时间和剂量相关性。对数据结果整理发现，在时间相关性方面，随药物作用时间的延长，乳腺癌细胞株MCF-7对脂类的利用率下降，细胞中脂类含量增加，同时肿瘤细胞分裂增殖受阻，细胞内DNA和RNA的含量降低，实验中观察到，48 h内，乳腺癌细胞MCF-7的红外光谱中代表脂类变化的峰强比 I2920/I1460逐渐升高， I1400/I1460逐渐降低，代表细胞内核酸变化的峰强比I1080/I1550， I1240/I1550逐渐降低；在剂量相关性方面，癌细胞红外光谱中峰强比 I1640/I1550较正常升高，实验中观察到峰强比 I1640/I1550随药物浓度的增加而呈现逐渐降低的趋势。以上这些变化与乳腺癌细胞在化疗药物干预过程中的生物学改变相符，可作为FTIR实时监测乳腺癌细胞化疗反应性的参考指标。
응용부리협변환홍외광보기술감측유선암세포주 MCF-7재화료약물5불뇨밀정（5-FU ）간예과정중상응적홍외광보변화，험증기여약물작용적시간화제량상관성。대수거결과정리발현，재시간상관성방면，수약물작용시간적연장，유선암세포주MCF-7대지류적이용솔하강，세포중지류함량증가，동시종류세포분렬증식수조，세포내DNA화RNA적함량강저，실험중관찰도，48 h내，유선암세포MCF-7적홍외광보중대표지류변화적봉강비 I2920/I1460축점승고， I1400/I1460축점강저，대표세포내핵산변화적봉강비I1080/I1550， I1240/I1550축점강저；재제량상관성방면，암세포홍외광보중봉강비 I1640/I1550교정상승고，실험중관찰도봉강비 I1640/I1550수약물농도적증가이정현축점강저적추세。이상저사변화여유선암세포재화료약물간예과정중적생물학개변상부，가작위FTIR실시감측유선암세포화료반응성적삼고지표。
The purpose of the present study was to compare the changes of infrared spectrum of breast cancer cell line MCF-7 treated by 5-fluorouracil with various concentrations and treating time .The results indicate that the relative intensity ratios of I2920/I1460 increased in the FTIR spectra of cell line MCF-7 while I1400/I1460 ,I1080/I1550 and I1240/I1550 decreased significantly within forty-eight hours ,however ,no regular change was observed during 48~72 hours .The relative intensity ratio of I1 640/I1 550 decreases along with the increase in the concentration of 5-fluorouracil .The changes of infrared spectra are consistent with the biochemical changes in breast cancer cell ,and it can be the reference designators when assessing curative effect of breast cancer cell by FTIR spectrometry .