CAJ | 학술논문

目的：研究鱼藤酮对大鼠脑内多巴胺能神经元的毒性作用机制。方法健康成年雄性Wistar大鼠背部皮下注射鱼藤酮制备帕金森病动物模型。采用免疫细胞化学、透射电镜技术及分光光度法技术检测大鼠脑内多巴胺能神经元的损伤及纹状体中氧化应激参数的改变。结果鱼藤酮组大鼠中脑酪氨酸羟化酶（TH）免疫反应阳性神经元数明显少于对照组（P＜0．01），纹状体中TH免疫反应强度明显降低（P＜0．05），透射电镜可见鱼藤酮组黑质多巴胺能神经元内线粒体损伤，树突变性，其内微粒、微管聚集，同时纹状体发生了明显的氧化损伤。结论氧化应激和超微结构改变是鱼藤酮对大鼠脑内多巴胺能神经元损伤的主要发病机制。
목적：연구어등동대대서뇌내다파알능신경원적독성작용궤제。방법건강성년웅성Wistar대서배부피하주사어등동제비파금삼병동물모형。채용면역세포화학、투사전경기술급분광광도법기술검측대서뇌내다파알능신경원적손상급문상체중양화응격삼수적개변。결과어등동조대서중뇌락안산간화매（TH）면역반응양성신경원수명현소우대조조（P＜0．01），문상체중TH면역반응강도명현강저（P＜0．05），투사전경가견어등동조흑질다파알능신경원내선립체손상，수돌변성，기내미립、미관취집，동시문상체발생료명현적양화손상。결론양화응격화초미결구개변시어등동대대서뇌내다파알능신경원손상적주요발병궤제。
Objective The study of the toxic mechanism about rotenone on dopaminergic neurons of brain in rats.Methods Healthy adult male Wistar rats were injected rotenone subcutaneous to prepare for Parkinson 's disease animal model.The damage of dopaminergic neurons of substantia nigra and striatum in rats brain was detected using immunocytochemistry , transmission electron microscopy and spectrophotometry .Results The TH immune response positive neuron numbers were significantly less in the midbrain of rotenone group as compared with control group ( P <0.01) .The TH strength of immune response in striatum significantly decreased in rotenone group ras ( P <0.05 ) .In the experimental group The microfilament microtubules gathered in the midbrain dopaminergic neurons, and oxidative damage significantly increased in striatum of rotenone group rats .Conclusions Oxidative stress and ultrastructure changes are the main pathogenesis of rotenone on dopaminergic neurons in rats .