CAJ | 학술논문

目的：观察尿畅舒胶囊对去势雌鼠膀胱质量、内皮型一氧化氮合酶(eNOS)及水通道蛋白1(AQP1)含量的影响，探讨其治疗绝经后膀胱过度活动症的机理。方法SD雌性大鼠随机分为空白组、模型组、尼尔雌醇组及尿畅舒组。摘除卵巢建立大鼠雌激素缺乏模型，各给药组给予相应药物灌胃，空白组及模型组灌胃等量生理盐水。给药4周后，测定膀胱质量、膀胱壁厚度；采用ELISA测定血清及膀胱组织中eNOS、AQP1的含量，分光光度法测定一氧化氮(NO)浓度。结果大鼠双侧卵巢切除后，膀胱质量减轻，膀胱黏膜层及肌层明显萎缩、变薄；尿畅舒组大鼠膀胱质量增加，黏膜层细胞排列紧密、层次分明，肌层增厚。空白组、尼尔雌醇组及尿畅舒组血清eNOS、NO均明显高于模型组(P＜0.05)；尿畅舒组与尼尔雌醇组比较，差异有统计学意义(P＜0.05)；去势大鼠血清AQP1明显下降，给予雌激素及尿畅舒胶囊后上升；而膀胱AQP1含量在去势前后及给予雌激素、尿畅舒胶囊后差异无统计学意义。结论尿畅舒胶囊可逆转雌激素缺乏导致的膀胱黏膜及平滑肌萎缩，提高血清及膀胱eNOS含量，从而治疗绝经后膀胱过度活动症。
목적：관찰뇨창서효낭대거세자서방광질량、내피형일양화담합매(eNOS)급수통도단백1(AQP1)함량적영향，탐토기치료절경후방광과도활동증적궤리。방법SD자성대서수궤분위공백조、모형조、니이자순조급뇨창서조。적제란소건립대서자격소결핍모형，각급약조급여상응약물관위，공백조급모형조관위등량생리염수。급약4주후，측정방광질량、방광벽후도；채용ELISA측정혈청급방광조직중eNOS、AQP1적함량，분광광도법측정일양화담(NO)농도。결과대서쌍측란소절제후，방광질량감경，방광점막층급기층명현위축、변박；뇨창서조대서방광질량증가，점막층세포배렬긴밀、층차분명，기층증후。공백조、니이자순조급뇨창서조혈청eNOS、NO균명현고우모형조(P＜0.05)；뇨창서조여니이자순조비교，차이유통계학의의(P＜0.05)；거세대서혈청AQP1명현하강，급여자격소급뇨창서효낭후상승；이방광AQP1함량재거세전후급급여자격소、뇨창서효낭후차이무통계학의의。결론뇨창서효낭가역전자격소결핍도치적방광점막급평활기위축，제고혈청급방광eNOS함량，종이치료절경후방광과도활동증。
Objective To explore the mechanism of Niaochangshu capsule in the treatment of postmenopausal overactive bladder, through observing its influence on bladder weight and the expression of eNOS and AQP1 of ovariectomized female rats. Methods Female SD rats were divided into blank group, model group, Nilestriol group and Niaochangshu group. Rats were removed ovaries except the blank group. The treatment groups were given corresponding drugs, blank group and model group were given normal saline by gavage. After 4 weeks, the bladders' weight and thickness were detected, the expressions of eNOS and AQP1 in serum and bladder tissue were determined by ELISA, and NO by spectrophotometry. Results Ovariectomy resulted in decreased bladder weight, bladder mucosal and muscular atrophy, and opposite changes showed after given Niaochangshu. The expressions of eNOS and NO in bladder and serum were decreased significantly after ovariectomy, while increased by given Niaochangshu capsule or nylestriol (P<0.05), and there was significant difference between Niaochangshu group and Nilestriol group (P<0.05). The expression of AQP1 was decreased in the model group, and increased after given nylestriol or Niaochangshu capsule. While the expression of AQP1 in bladder had no significant difference among the four groups. Conclusion Niaochangshu capsule can reverse bladder mucosal and muscular atrophy caused by estrogen deficiency, and increase the content of eNOS in serum and bladder, thus play the role in the treatment of postmenopausal overactive bladder.