CAJ | 학술논문

目的：观察紫癜汤及其拆方对特发性血小板减少性紫癜(ITP)模型小鼠外周血网织血小板(RP)、血清中血小板生成素(TPO)的影响,探讨该方提升ITP模型小鼠血小板的作用机制及其核心结构。方法将84只BalB/c 小鼠随机分为正常组、模型组、泼尼松组、紫癜汤组、补益气血组、补气活血组、补血活血组。除正常组外,其余各组采用免疫法腹腔注射豚鼠抗小鼠血小板血清建立ITP小鼠模型。于造模第5日起灌胃给药,正常组、模型组给予生理盐水0.2 mL/只,紫癜汤组给予紫癜汤0.43 mL/只,补益气血组给予补益气血汤0.36 mL/只,补气活血组给予补气活血汤0.27 mL/只,补血活血组给予补血活血汤0.3 mL/只,泼尼松组给予醋酸泼尼松混悬液0.2 mL/只,连续10 d。眼球取血分离血清,检测小鼠外周血小板、RP及TPO。结果各给药组均能提高ITP小鼠血小板计数,与给药前比较,紫癜汤组、补气活血组、泼尼松组差异有统计学意义(P＜0.05)；模型组 RP显著高于正常组,RP绝对值低于正常组(P＜0.05)。各给药组 RP降低,与模型组比较差异有统计学意义(P＜0.05),且RP与血小板计数呈负相关(P＜0.05)。模型组血清中TPO水平高于正常组(P＜0.05)；各给药组TPO水平下降,与模型组比较差异有统计学意义(P＜0.05)。结论紫癜汤的核心结构是补气药、活血药,其能有效提升ITP模型小鼠血小板的机制与降低外周血RP及血清中TPO有关。
목적：관찰자전탕급기탁방대특발성혈소판감소성자전(ITP)모형소서외주혈망직혈소판(RP)、혈청중혈소판생성소(TPO)적영향,탐토해방제승ITP모형소서혈소판적작용궤제급기핵심결구。방법장84지BalB/c 소서수궤분위정상조、모형조、발니송조、자전탕조、보익기혈조、보기활혈조、보혈활혈조。제정상조외,기여각조채용면역법복강주사돈서항소서혈소판혈청건립ITP소서모형。우조모제5일기관위급약,정상조、모형조급여생리염수0.2 mL/지,자전탕조급여자전탕0.43 mL/지,보익기혈조급여보익기혈탕0.36 mL/지,보기활혈조급여보기활혈탕0.27 mL/지,보혈활혈조급여보혈활혈탕0.3 mL/지,발니송조급여작산발니송혼현액0.2 mL/지,련속10 d。안구취혈분리혈청,검측소서외주혈소판、RP급TPO。결과각급약조균능제고ITP소서혈소판계수,여급약전비교,자전탕조、보기활혈조、발니송조차이유통계학의의(P＜0.05)；모형조 RP현저고우정상조,RP절대치저우정상조(P＜0.05)。각급약조 RP강저,여모형조비교차이유통계학의의(P＜0.05),차RP여혈소판계수정부상관(P＜0.05)。모형조혈청중TPO수평고우정상조(P＜0.05)；각급약조TPO수평하강,여모형조비교차이유통계학의의(P＜0.05)。결론자전탕적핵심결구시보기약、활혈약,기능유효제승ITP모형소서혈소판적궤제여강저외주혈RP급혈청중TPO유관。
Objective To observe the effects of Purpura decoction and its disassembled prescriptions on peripheral blood reticulated platelets (RP) and serum thrombopoietin (TPO) of idiopathic thrombocytopenic purpura (ITP) model mice, to explore the action mechanism and core structure of the prescription. Methods Totally 84 BalB/c mice were randomly divided into normal group, model group, prednisone group, Purpura decoction group, Buyi Qixue group, Buqi Huoxue group and Buxue Huoxue group. ITP mouse model was made by injecting intraperitoneally guinea pig-antimouse platelet serum. From the 5th day of modeling, mice were intragastrically administered. The normal group and model group were fed with normal saline 0.2 mL per mouse. Purpura decoction group was fed with Purpura decoction 0.43 mL per mouse. Buyi Qixue group was fed with Buyi Qixue decoction 0.36 mL per mouse. Buqi Huoxue group was fed with Buqi Huoxue decoction 0.27 mL per mouse. Buxue Huoxue group was fed with Buxue Huoxue decoction 0.3 mL per mouse. Prednisone group was fed with prednisone acetate suspension 0.2 mL per mouse. Ten days later, took the blood from the eyeball and isolated serum, the mouse peripheral platelet, RP and TPO were observed. Results After treatment, the platelet count of treatment groups was improved. Compared with before treatment, Purpura decoction group,Buqi Huoxue group and prednisone group had significant difference in platelet count (P<0.05). The RP of model group was significantly higher than the normal group (P<0.05), but RP absolute value was lower than the normal group (P<0.05). Compared with the model group, RP of treatment groups reduced (P<0.05), and RP was negatively correlated with platelet count (P<0.05). TPO level of the model group was higher than that of the normal group (P<0.01). TPO of treatment groups declined significantly, and had significant differences with that of the model group (P<0.05). Conclusion Core structure of Purpura decoction is qi tonifying and blood activating drug. The mechanism of treating ITP is related with lowering RP and TPO.