CAJ | 학술논문

目的：观察五子衍宗方对环磷酰胺(CTX)致小鼠 DNA 损伤的影响,并探讨其作用机制。方法将BalB/c 小鼠随机分为正常组、模型组、五子衍宗方低剂量组、五子衍宗方高剂量组。五子衍宗方低、高剂量组预给药1周后,除正常组外,其余各组腹腔注射CTX 100 mg/kg造模,隔日1次,共3次,同时五子衍宗方低、高剂量组继续给药,末次给予CTX后12 h处死小鼠,分离血清,ELISA检测血清中8-羟基脱氧鸟苷(8-OHdG)的含量,应用单细胞凝胶电泳技术检测骨髓细胞DNA损伤。结果五子衍宗方低、高剂量组能够降低血清中8-OHdG的含量,使小鼠骨髓细胞彗星图像的Olive尾矩、尾距、尾长和尾部DNA含量降低。结论五子衍宗方对CTX致小鼠DNA损伤具有较好的保护作用。
목적：관찰오자연종방대배린선알(CTX)치소서 DNA 손상적영향,병탐토기작용궤제。방법장BalB/c 소서수궤분위정상조、모형조、오자연종방저제량조、오자연종방고제량조。오자연종방저、고제량조예급약1주후,제정상조외,기여각조복강주사CTX 100 mg/kg조모,격일1차,공3차,동시오자연종방저、고제량조계속급약,말차급여CTX후12 h처사소서,분리혈청,ELISA검측혈청중8-간기탈양조감(8-OHdG)적함량,응용단세포응효전영기술검측골수세포DNA손상。결과오자연종방저、고제량조능구강저혈청중8-OHdG적함량,사소서골수세포혜성도상적Olive미구、미거、미장화미부DNA함량강저。결론오자연종방대CTX치소서DNA손상구유교호적보호작용。
Objective To study protective effects of Wuzi Yanzong Fang on DNA damage induced by cyclophosphamide (CTX) in mice, and explore its mechanism. Methods BalB/c mice were randomly divided into normal group, model group, Wuzi Yanzong Fang low dose group and Wuzi Yanzong Fang high dose group. Mice in Wuzi Yanzong Fang groups were pretreated with Wuzi Yanzong Fang for 7 days, then the mice in Wuzi Yanzong Fang groups and model group were intraperitoneally injected with CTX (100 mg/kg) every other day for three times, and mice in Wuzi Yanzong Fang groups were continued administered with Wuzi Yanzong Fang. Animals were sacrificed in twelve hours after the final treatment of CTX. ELISA was used to detect 8-OHdG content in serum, and single cell gel electrophoresis to detect DNA damage in bone marrow cells. Results Wuzi Yanzong Fang low dose group and high dose group reduced the level of 8-OHdG in serum. Wuzi Yanzong Fang significantly decreased Olive tail moment, tail moment, tail length and tail DNA%in mouse bone marrow cells. Conclusion Wuzi Yanzong Fang has good protective effects on DNA damage caused by CTX.