CAJ | 학술논문

乳清是生产干酪及干酪素过程中获得的大量液态副产物，含有丰富的营养成分，其主要成分乳清蛋白的营养价值极高。随着近年来小肽生物活性功能研究的开展，对于乳清蛋白水解物中功能性多肽的研究成为主要方向。近年来的研究中发现乳清蛋白中含有多种生物活性肽，包括 ACE 抑制肽、抑菌肽、抗病毒肽、抗氧化活性肽及其它活性肽。目前，国内外有关 ACE 抑制肽的研究较多，而对降胆固醇肽的研究多是以大豆蛋白为原料，而更具有研究价值的乳清蛋白源降胆固醇肽研究较少。本人以乳清蛋白为原料，对其酶解产物的降胆固醇活性肽进行分离制备，既充分利用了干酪的副产物，减少了资源浪费和环境污染，又能提供一种新型降胆固醇功能产品，为商业化开发乳清蛋白降胆固醇功能产品提供理论依据。本文结合本人对降胆固醇肽的研究，综述了乳清蛋白源功能性多肽的制备、分离纯化技术及其活性多肽的研究概况。
유청시생산간락급간락소과정중획득적대량액태부산물，함유봉부적영양성분，기주요성분유청단백적영양개치겁고。수착근년래소태생물활성공능연구적개전，대우유청단백수해물중공능성다태적연구성위주요방향。근년래적연구중발현유청단백중함유다충생물활성태，포괄 ACE 억제태、억균태、항병독태、항양화활성태급기타활성태。목전，국내외유관 ACE 억제태적연구교다，이대강담고순태적연구다시이대두단백위원료，이경구유연구개치적유청단백원강담고순태연구교소。본인이유청단백위원료，대기매해산물적강담고순활성태진행분리제비，기충분이용료간락적부산물，감소료자원낭비화배경오염，우능제공일충신형강담고순공능산품，위상업화개발유청단백강담고순공능산품제공이론의거。본문결합본인대강담고순태적연구，종술료유청단백원공능성다태적제비、분리순화기술급기활성다태적연구개황。
With the development of research on biological activity of small peptides in recent years, re-search on functions of whey protein hydrolysate peptides become hot. Whey is a liquid by-product obtained in the process of cheese and casein. Whey is rich in nutrients, and its main component is whey protein which has a high nutritional value. Recent studies have found that whey protein contains a variety of bioactive peptides, in-cluding antihypertensive peptides, antimicrobial peptides, antiviral peptide, antioxidant activity peptides and other bioactive peptides, and has been a hot research. At present, there are many studies on the antihypertensive peptides at home and abroad, and there are fewer studies on hypocholesterolemic peptides. Most of studies about hypocholesterolemic peptides are about soy protein. However, whey protein hypocholesterolemic pep-tides have more research value, in fact. In this experiment, whey protein was as raw material, hypocholestero-lemic peptides were separated and prepared from its hydrolysates. In this subject, by-product of cheese was full used, it not only reduced waste of resources and environmental pollution, but also provided a theoretical basis for the commercial development of hypocholesterolemic functional products. This paper reviews the whey protein source of functional polypeptides preparation, separation and purification technology and research status of active peptides.