中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
6期
83-85
,共3页
尿塞通胶囊%丹参素钠%橙皮苷%丹酚酸B%高效液相色谱法%含量测定
尿塞通膠囊%丹參素鈉%橙皮苷%丹酚痠B%高效液相色譜法%含量測定
뇨새통효낭%단삼소납%등피감%단분산B%고효액상색보법%함량측정
Niaosaitong Capsules%sodium Danshensu%hesperidin%salvianolic acid B%HPLC%content determination
目的：建立同时测定尿塞通胶囊中丹参素钠、橙皮苷、丹酚酸B含量的方法。方法采用高效液相色谱法,C18色谱柱,以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长286 nm。结果丹参素钠、橙皮苷、丹酚酸 B 分别在0.03844～1.922μg、0.0002070528～4.044μg、0.03428～1.714μg范围内线性关系良好,平均回收率为90.9%～107.1%,RSD为2.1%～3.2%。结论所建立的方法简便、准确,可用于尿塞通胶囊的质量控制。
目的：建立同時測定尿塞通膠囊中丹參素鈉、橙皮苷、丹酚痠B含量的方法。方法採用高效液相色譜法,C18色譜柱,以乙腈-0.1%燐痠為流動相進行梯度洗脫,流速1.0 mL/min,柱溫30℃,檢測波長286 nm。結果丹參素鈉、橙皮苷、丹酚痠 B 分彆在0.03844～1.922μg、0.0002070528～4.044μg、0.03428～1.714μg範圍內線性關繫良好,平均迴收率為90.9%～107.1%,RSD為2.1%～3.2%。結論所建立的方法簡便、準確,可用于尿塞通膠囊的質量控製。
목적：건립동시측정뇨새통효낭중단삼소납、등피감、단분산B함량적방법。방법채용고효액상색보법,C18색보주,이을정-0.1%린산위류동상진행제도세탈,류속1.0 mL/min,주온30℃,검측파장286 nm。결과단삼소납、등피감、단분산 B 분별재0.03844～1.922μg、0.0002070528～4.044μg、0.03428～1.714μg범위내선성관계량호,평균회수솔위90.9%～107.1%,RSD위2.1%～3.2%。결론소건립적방법간편、준학,가용우뇨새통효낭적질량공제。
Objectives To establish an HPLC method for determination of sodium Danshensu, hesperidin and salvianolic acid B in Niaosaitong capsules. Methods Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength was set at 286 nm. Results Sodium Danshensu, hesperidin and salvianolic acid B had good linearity in the range of 0.038 44-1.922 μg, 0.000 207 052 8-4.044 μg, 0.034 28-1.714 μg respectively, and the average recovery rate was 90.9%-107.1%, RSD was 2.1%-3.2%. Conclusion The methods is simple and accurate, and can be used for the quality control of Niaosaitong capsules.
