中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
6期
72-75
,共4页
马宁%朱旭江%杨锡%王兰霞
馬寧%硃旭江%楊錫%王蘭霞
마저%주욱강%양석%왕란하
仁青芒觉胶囊%质量标准%薄层色谱法%高效液相色谱法%马钱子碱%士的宁
仁青芒覺膠囊%質量標準%薄層色譜法%高效液相色譜法%馬錢子堿%士的寧
인청망각효낭%질량표준%박층색보법%고효액상색보법%마전자감%사적저
Renqing Mangjue capsules%quality standard%TLC%HPLC%Brucine%Strychnine
目的：制定仁青芒觉胶囊的质量标准。方法采用薄层色谱法对西红花、姜黄、丁香、木香、冰片进行定性鉴别。采用高效液相色谱法,CAPCELL PAK MG C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇∶0.01 moL/L磷酸二氢钾溶液(用10%磷酸调节pH值2.5)＝25∶75,流速1.0 mL/min,检测波长为254、260 nm,柱温30℃,对马钱子碱、士的宁进行定量测定。结果薄层色谱能定性检出西红花、姜黄、丁香、木香、冰片,斑点清晰,且阴性对照无干扰。马钱子碱在0.0121～0.0728μg范围内呈良好的线性关系,r＝0.9991,加样回收率为97.27%,RSD＝1.20%；士的宁在0.0454～0.2724μg范围内呈良好的线性关系,r＝0.9998,加样回收率为98.69%,RSD＝1.17%。结论该方法操作简便、专属性、重复性好,可有效控制仁青芒觉胶囊的质量。
目的：製定仁青芒覺膠囊的質量標準。方法採用薄層色譜法對西紅花、薑黃、丁香、木香、冰片進行定性鑒彆。採用高效液相色譜法,CAPCELL PAK MG C18色譜柱(4.6 mm×250 mm,5μm),流動相為甲醇∶0.01 moL/L燐痠二氫鉀溶液(用10%燐痠調節pH值2.5)＝25∶75,流速1.0 mL/min,檢測波長為254、260 nm,柱溫30℃,對馬錢子堿、士的寧進行定量測定。結果薄層色譜能定性檢齣西紅花、薑黃、丁香、木香、冰片,斑點清晰,且陰性對照無榦擾。馬錢子堿在0.0121～0.0728μg範圍內呈良好的線性關繫,r＝0.9991,加樣迴收率為97.27%,RSD＝1.20%；士的寧在0.0454～0.2724μg範圍內呈良好的線性關繫,r＝0.9998,加樣迴收率為98.69%,RSD＝1.17%。結論該方法操作簡便、專屬性、重複性好,可有效控製仁青芒覺膠囊的質量。
목적：제정인청망각효낭적질량표준。방법채용박층색보법대서홍화、강황、정향、목향、빙편진행정성감별。채용고효액상색보법,CAPCELL PAK MG C18색보주(4.6 mm×250 mm,5μm),류동상위갑순∶0.01 moL/L린산이경갑용액(용10%린산조절pH치2.5)＝25∶75,류속1.0 mL/min,검측파장위254、260 nm,주온30℃,대마전자감、사적저진행정량측정。결과박층색보능정성검출서홍화、강황、정향、목향、빙편,반점청석,차음성대조무간우。마전자감재0.0121～0.0728μg범위내정량호적선성관계,r＝0.9991,가양회수솔위97.27%,RSD＝1.20%；사적저재0.0454～0.2724μg범위내정량호적선성관계,r＝0.9998,가양회수솔위98.69%,RSD＝1.17%。결론해방법조작간편、전속성、중복성호,가유효공제인청망각효낭적질량。
Objective To set up the quality standard of Renqing Mangjue capsules. Methods TLC was used for qualitative identification of Crocus sativus, Zingiberaceae, Syringa oblata, Radix Aucklandiae and Borneolum. The content of Brucine and Strychnine were determined by HPLC, which conducted with CAPCELL PAK MG C18 column (4.6 mm×250 mm, 5μm). The mobile phase consisted of methanol∶0.01 moL/L potassium dihydrogen phosphate (with 10%phosphoric acid to adjust the pH 2.5)=25∶75 and the flow rate was 1.0 mL/min. The detection wavelength was set at 254 nm and 260 nm. The column temperature maintained at 30 ℃. Results TLC could detect qualitatively Crocus sativus, Zingiberaceae, Syringa oblata, Radix Aucklandiae and Borneolum. The spots were clear and the negative controls had no interference. HPLC determined that Brucine presented a good linear relationship in the range of 0.012 1-0.072 8 μg (r=0.999 1). The average recovery was 97.27%, RSD was 1.20%. Strychnine presented a good linear relationship in the range of 0.045 4-0.272 4 μg (r=0.999 8). The average recovery was 98.69%, RSD was 1.17%. Conclusion The method is simple in operation. The results are accurate, reliable and good in reproducibility. The method can effectively control the quality of Renqing Mangjue capsules.
