CAJ | 학술논문

目的：制备银溶胶表面增强拉曼散射(surface-enhanced Raman scattering, SERS)活性基底,并基于自行制备的基底检测米线中的乌洛托品。方法利用扫描电子显微镜(SEM)和紫外-可见吸收光谱对制备的银纳米颗粒进行表征。采用氯化钠作为促凝剂,对所制备的活性基底进行性能优化。基于自行制备的SERS活性基底,研究了乌洛托品的表面增强拉曼光谱,并将这种检测方法应用于米线中乌洛托品的检测。结果当乌洛托品的浓度达到1 mg/kg时,仍然可以得到明显的拉曼信号,乌洛托品在1~5 mg/kg 的范围内呈现良好的线性关系, R2=0.928。结论 SERS技术操作简便、快速、准确,可用于米线中痕量乌洛托品的现场快速检测。
목적：제비은용효표면증강랍만산사(surface-enhanced Raman scattering, SERS)활성기저,병기우자행제비적기저검측미선중적오락탁품。방법이용소묘전자현미경(SEM)화자외-가견흡수광보대제비적은납미과립진행표정。채용록화납작위촉응제,대소제비적활성기저진행성능우화。기우자행제비적SERS활성기저,연구료오락탁품적표면증강랍만광보,병장저충검측방법응용우미선중오락탁품적검측。결과당오락탁품적농도체도1 mg/kg시,잉연가이득도명현적랍만신호,오락탁품재1~5 mg/kg 적범위내정현량호적선성관계, R2=0.928。결론 SERS기술조작간편、쾌속、준학,가용우미선중흔량오락탁품적현장쾌속검측。
Objective Silver colloid was prepared and it was used to detect urotropine in rice noodle. Methods Silver colloid was characterized by scanning electronic microscopy(SEM)and UV-vis absorption spectra. In order to improve the colloid, NaCl was used as aggregating agent. The Raman surface-enhanced scattering (SERS) spectra of urotropine were investigated based on the self-prepared colloid. SERS was used to detect urotropine in rice noodle. Results The limit of qualitative detection was 1 mg/kg. The method showed a good linearity in the range of 1~5 mg/kg for urotropine with R2=0.928. Conclusion This method has the advantages of simple pretreatment, low detection limit and it is suitable for the fast determination of urotropine in rice noodle.