中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2014年
3期
44-46
,共3页
脓毒症%100％氧气%胆碱能抗炎通路%α7烟碱型乙酰胆碱受体(α7nAchR)%肿瘤坏死因子α
膿毒癥%100％氧氣%膽堿能抗炎通路%α7煙堿型乙酰膽堿受體(α7nAchR)%腫瘤壞死因子α
농독증%100％양기%담감능항염통로%α7연감형을선담감수체(α7nAchR)%종류배사인자α
目的：观察小鼠皮下注射α7烟碱型乙酰胆碱受体（α7nAchR）拮抗剂甲基牛扁亭对100%氧吸入后酵母多糖所诱导的脓毒症小鼠的变化及其意义。方法：腹腔注射酵母多糖或等容量生理盐水，随机分组为：(1)生理盐水+空气；(2)酵母多糖+空气；(3)酵母多糖+空气+甲基牛扁亭组；(4)酵母多糖+100%氧气；(5)酵母多糖+100%氧气+甲基牛扁亭组。观察7天动物存活率，注射酵母多糖后24小时取心、肝、肺、肾，做组织病理学以及病理学评分，测定15小时血气和24小时血浆中生化指标及炎症因子。结果：100%氧吸入可提高脓毒症动物的存活率，改善其肝肾生化指标，降低病理评分，减低炎症因子T N F水平。给予α7nAchR受体拮抗剂甲基牛扁亭后，脓毒症动物第7天存活率由80%下降到40%，各器官病理评分显著升高，测定血浆中各项生化指标显著增高，血浆中肿瘤坏死因子-α水平显著增高，且与脓毒症+100%氧气组比较，其差异均有显著统计学意义(p<0.05)。结论：100%氧对酵母多糖所致脓毒症小鼠有保护作用。α7nAchR受体拮抗剂甲基牛扁亭可逆转100%氧气对酵母多糖所致脓毒症小鼠的保护作用，表明胆碱能抗炎通路参与100%氧对酵母多糖所致脓毒症小鼠的保护效应。
目的：觀察小鼠皮下註射α7煙堿型乙酰膽堿受體（α7nAchR）拮抗劑甲基牛扁亭對100%氧吸入後酵母多糖所誘導的膿毒癥小鼠的變化及其意義。方法：腹腔註射酵母多糖或等容量生理鹽水，隨機分組為：(1)生理鹽水+空氣；(2)酵母多糖+空氣；(3)酵母多糖+空氣+甲基牛扁亭組；(4)酵母多糖+100%氧氣；(5)酵母多糖+100%氧氣+甲基牛扁亭組。觀察7天動物存活率，註射酵母多糖後24小時取心、肝、肺、腎，做組織病理學以及病理學評分，測定15小時血氣和24小時血漿中生化指標及炎癥因子。結果：100%氧吸入可提高膿毒癥動物的存活率，改善其肝腎生化指標，降低病理評分，減低炎癥因子T N F水平。給予α7nAchR受體拮抗劑甲基牛扁亭後，膿毒癥動物第7天存活率由80%下降到40%，各器官病理評分顯著升高，測定血漿中各項生化指標顯著增高，血漿中腫瘤壞死因子-α水平顯著增高，且與膿毒癥+100%氧氣組比較，其差異均有顯著統計學意義(p<0.05)。結論：100%氧對酵母多糖所緻膿毒癥小鼠有保護作用。α7nAchR受體拮抗劑甲基牛扁亭可逆轉100%氧氣對酵母多糖所緻膿毒癥小鼠的保護作用，錶明膽堿能抗炎通路參與100%氧對酵母多糖所緻膿毒癥小鼠的保護效應。
목적：관찰소서피하주사α7연감형을선담감수체（α7nAchR）길항제갑기우편정대100%양흡입후효모다당소유도적농독증소서적변화급기의의。방법：복강주사효모다당혹등용량생리염수，수궤분조위：(1)생리염수+공기；(2)효모다당+공기；(3)효모다당+공기+갑기우편정조；(4)효모다당+100%양기；(5)효모다당+100%양기+갑기우편정조。관찰7천동물존활솔，주사효모다당후24소시취심、간、폐、신，주조직병이학이급병이학평분，측정15소시혈기화24소시혈장중생화지표급염증인자。결과：100%양흡입가제고농독증동물적존활솔，개선기간신생화지표，강저병리평분，감저염증인자T N F수평。급여α7nAchR수체길항제갑기우편정후，농독증동물제7천존활솔유80%하강도40%，각기관병리평분현저승고，측정혈장중각항생화지표현저증고，혈장중종류배사인자-α수평현저증고，차여농독증+100%양기조비교，기차이균유현저통계학의의(p<0.05)。결론：100%양대효모다당소치농독증소서유보호작용。α7nAchR수체길항제갑기우편정가역전100%양기대효모다당소치농독증소서적보호작용，표명담감능항염통로삼여100%양대효모다당소치농독증소서적보호효응。
Objective: Studies have shown that 100% oxygen inhalation has a protective effect on murine sepsis model, without causing oxidative stress and organ damage, suggesting that high oxygen may become an important treatment for sepsis. This article was scheduled to observe the effects of subcutaneously injected α7 nicotinic acetylcholine receptor (α7nAchR) antagonist methyl ycaconitine on protective action against zymosan-induced sepsis by 100% oxygen in mice. Methods: Animals having intraperitoneal injection of zymosan or the same volume of normal saline, were randomly divided into five groups: (1) normal saline + air; (2) zymosan + air; (3) zymosan + air + methyl ycaconitine; (4) zymosan +100% oxygen; (5) zymosan+100% oxygen + methyl ycaconitine groups. 7-day animal survival rate, heart, liver, lung, kidney tissue pathology and pathological scores as wel as plasma biochemical indices and inflammatory factors at 24 hours after zymosan injection were determined. Results: 100% oxygen inhalation improved the survival rate of animals with sepsis, improved liver and kidney biochemical indicators, and reduced the pathologic score and the level of inflammatory cytokine TNF. Given α7nAchR receptor antagonist methyl ycaconitine, the7-day animal survival rate decreased from 80% to 40%, organ pathology score was significantly increased, measured plasma biochemical indexes and plasma tumor necrosis factor-α levels were significantly increased, when compared with those in the zymosan +100% oxygen group, with statistical y significant differences (P <0.05). Conclusion: 100% oxygen has a protective effect on zymosan induced septic mice which was reversed byα7nAchR antagonist, indicating that the cholinergic anti-inflammatory pathway is involved in the protective effect of 100% oxygen on zymosan-induced sepsis.
