CAJ | 학술논문

研究饲料中添加乌贼肝粉和鱼浆蛋白对中华鳖生长和肝功能的影响.共设计3个实验组,即对照组(白鱼粉组)、乌贼肝粉组和鱼浆蛋白组.3种实验饲料的营养组成相近,其中乌贼肝粉组和鱼浆蛋白组的饲料分别用乌贼肝粉和鱼浆蛋白取代对照组饲料中5%的白鱼粉.用3种饲料分别养殖中华鳖稚鳖8周.结果发现,3个实验组稚鳖的摄食率和生长速度没有显著差异.乌贼肝粉组的饲料系数与对照组无显著差异,但鱼浆蛋白组的饲料系数显著高于对照组和乌贼肝粉组.乌贼肝粉组的干物质和脂肪消化率显著高于对照组,蛋白消化率与对照组无显著差异；鱼浆蛋白组的干物质、蛋白和脂肪消化率均显著高于对照组.乌贼肝粉组稚鳖的肝谷草转氨酶(glutamic-oxalacetic transminase,GOT)活性显著低于对照组,血清谷丙转氨酶活性(glutamic-pyruvic transminase,GPT)显著高于对照组.鱼浆蛋白组肝和血清的 GOT 和 GPT 活性与对照组均无显著差异.3个实验组稚鳖的血清三酰甘油和总胆固醇含量没有显著差异.实验结果表明,饲料中添加5%的乌贼肝粉有可能对稚鳖肝造成轻微损伤；饲料中添加5%的鱼浆蛋白将降低饲料的利用率,但对稚鳖肝功能没有显著影响.
연구사료중첨가오적간분화어장단백대중화별생장화간공능적영향.공설계3개실험조,즉대조조(백어분조)、오적간분조화어장단백조.3충실험사료적영양조성상근,기중오적간분조화어장단백조적사료분별용오적간분화어장단백취대대조조사료중5%적백어분.용3충사료분별양식중화별치별8주.결과발현,3개실험조치별적섭식솔화생장속도몰유현저차이.오적간분조적사료계수여대조조무현저차이,단어장단백조적사료계수현저고우대조조화오적간분조.오적간분조적간물질화지방소화솔현저고우대조조,단백소화솔여대조조무현저차이；어장단백조적간물질、단백화지방소화솔균현저고우대조조.오적간분조치별적간곡초전안매(glutamic-oxalacetic transminase,GOT)활성현저저우대조조,혈청곡병전안매활성(glutamic-pyruvic transminase,GPT)현저고우대조조.어장단백조간화혈청적 GOT 화 GPT 활성여대조조균무현저차이.3개실험조치별적혈청삼선감유화총담고순함량몰유현저차이.실험결과표명,사료중첨가5%적오적간분유가능대치별간조성경미손상；사료중첨가5%적어장단백장강저사료적이용솔,단대치별간공능몰유현저영향.
and liver function of P.sinensis . <br> Three experimental groups were designed,control group(white fishmeal group,WFM),squid liver meal group (SLM)and soluble fish protein concentrates group(SFP).Nutritional compositions of three diets were similar.For the diets of SLM and SFP,5% white fishmeal in the control diet was replaced with squid liver meal and soluble fish protein concentrates respectively.The feeding experiment lasted for eight weeks.Chinese turtles with initial average body mass of 6.8 g were fed twice a day at(30±1) ℃.Feed intake,growth and feed utilization were determined, and the activities of glutamic-oxalacetic transminase(GOT) and glutamic-pyruvic transminase (GPT) for liver and serum and the contents of serum triglyceride and cholesterol were analyzed. <br> No significant difference was found in feeding rate,specific growth rate,viscera-somatic index,hepatosomatic index among three experimental groups.Feed coefficient of SFP was significantly higher than WFM and SLM,and there was no significant difference in feed coefficient between WFM and SLM.Digestibilities of dry mass and lipid of SLM were both significantly higher than those of WFM.No difference in protein digestibility was found between SLM and WFM.Digestibilities of dry mass,protein and lipid of SFP were all significantly higher than those of WFM.Hepatic GOT activity of SLM was significantly lower than that of WFM,and serum GPT activity of SLM was significantly higher than that of WFM.There were no significant differences in GPT and GOT activities between SFP and WFM for both liver and serum tissues.No significant difference was found in the contents of serum triglyceride and cholesterol among three experimental groups. <br> These results indicated that 5% dietary supplementation of squid liver meal may cause slight hepatic damage to turtle,but had no evident effect on growth;and 5% dietary supplementation of soluble fish protein concentrates could reduce feed utilization,but had no adverse effects on liver function of turtle.Since the price of squid liver meal is lower than that of soluble fish protein concentrates,squid liver meal may be more suitable than soluble fish protein concentrates to replace fishmeal in turtle feed,and the recommended supplementation level should be less than 5%.