中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
4期
251-255
,共5页
张传领%葛玉梅%沈丽芳%陈琴琴%孙爱华%严杰
張傳領%葛玉梅%瀋麗芳%陳琴琴%孫愛華%嚴傑
장전령%갈옥매%침려방%진금금%손애화%엄걸
志贺菌%耐药性%超广谱β-内酰胺酶%AmpC酶%基因型
誌賀菌%耐藥性%超廣譜β-內酰胺酶%AmpC酶%基因型
지하균%내약성%초엄보β-내선알매%AmpC매%기인형
Shigella%Resistance%Extended-spectrum β-lactamases%AmpCβ-lactamases%Genotype
目的：确定头孢西丁耐药志贺菌临床菌株超广谱β-内酰胺酶（ ESBLs ）和头孢菌素酶（ AmpC）表型、基因型及毒力特征。方法 K-B扩散法筛选头孢西丁耐药志贺菌临床菌株并进行血清学分型。采用ESBLs确证试验和AmpC三维试验确定产酶表型， PCR及序列分析确定其ESBLs 和AmpC基因型。采用PCR检测上述志贺菌株毒力基因virA、ial、ipaH、set1A、set1B和sen，以确定其毒力基因型。结果从356株志贺菌临床菌株中检出8株头孢西丁耐药菌株，其中6株为福氏志贺菌（4株F2a、1株F2b、1株F2c）、2株为宋内志贺菌。8株头孢西丁耐药志贺菌中，5株ESBLs确证试验阳性，另2株AmpC酶三维试验阳性；7株携带ESBLs基因（ CTX-M-14、15、57和/或OXA-30），其中2株检出AmpC基因（ DHA-1和CMY-2）。8株头孢西丁耐药志贺菌中，5株携带所有6种受检的毒力基因，3株携带除set1A和set1B以外的4种毒力基因。两株同时产ESBLs和AmpC酶志贺菌仅对亚胺培南敏感。结论头孢西丁耐药志贺菌临床菌株产ESBLs为主且有较强毒力，但部分菌株同时产AmpC酶，其中DHA-1型AmpC酶为国内首次发现。
目的：確定頭孢西丁耐藥誌賀菌臨床菌株超廣譜β-內酰胺酶（ ESBLs ）和頭孢菌素酶（ AmpC）錶型、基因型及毒力特徵。方法 K-B擴散法篩選頭孢西丁耐藥誌賀菌臨床菌株併進行血清學分型。採用ESBLs確證試驗和AmpC三維試驗確定產酶錶型， PCR及序列分析確定其ESBLs 和AmpC基因型。採用PCR檢測上述誌賀菌株毒力基因virA、ial、ipaH、set1A、set1B和sen，以確定其毒力基因型。結果從356株誌賀菌臨床菌株中檢齣8株頭孢西丁耐藥菌株，其中6株為福氏誌賀菌（4株F2a、1株F2b、1株F2c）、2株為宋內誌賀菌。8株頭孢西丁耐藥誌賀菌中，5株ESBLs確證試驗暘性，另2株AmpC酶三維試驗暘性；7株攜帶ESBLs基因（ CTX-M-14、15、57和/或OXA-30），其中2株檢齣AmpC基因（ DHA-1和CMY-2）。8株頭孢西丁耐藥誌賀菌中，5株攜帶所有6種受檢的毒力基因，3株攜帶除set1A和set1B以外的4種毒力基因。兩株同時產ESBLs和AmpC酶誌賀菌僅對亞胺培南敏感。結論頭孢西丁耐藥誌賀菌臨床菌株產ESBLs為主且有較彊毒力，但部分菌株同時產AmpC酶，其中DHA-1型AmpC酶為國內首次髮現。
목적：학정두포서정내약지하균림상균주초엄보β-내선알매（ ESBLs ）화두포균소매（ AmpC）표형、기인형급독력특정。방법 K-B확산법사선두포서정내약지하균림상균주병진행혈청학분형。채용ESBLs학증시험화AmpC삼유시험학정산매표형， PCR급서렬분석학정기ESBLs 화AmpC기인형。채용PCR검측상술지하균주독력기인virA、ial、ipaH、set1A、set1B화sen，이학정기독력기인형。결과종356주지하균림상균주중검출8주두포서정내약균주，기중6주위복씨지하균（4주F2a、1주F2b、1주F2c）、2주위송내지하균。8주두포서정내약지하균중，5주ESBLs학증시험양성，령2주AmpC매삼유시험양성；7주휴대ESBLs기인（ CTX-M-14、15、57화/혹OXA-30），기중2주검출AmpC기인（ DHA-1화CMY-2）。8주두포서정내약지하균중，5주휴대소유6충수검적독력기인，3주휴대제set1A화set1B이외적4충독력기인。량주동시산ESBLs화AmpC매지하균부대아알배남민감。결론두포서정내약지하균림상균주산ESBLs위주차유교강독력，단부분균주동시산AmpC매，기중DHA-1형AmpC매위국내수차발현。
Objective To investigate the phenotypes and genotypes of extended -spectrum β-lacta-mases (ESBLs) and AmpC β-lactamases produced by cefoxitin-resistant Shigella strains isolated from Zhe-jiang province and the virulence of those strains .Methods Kirby-Bauer antibiotic testing was performed to screen cefoxitin-resistant strains from 356 Shigella isolates.The serotypes of the cefoxitin-resistant strains were detected .The phenotypes of ESBLs and AmpC β-lactamases from cefoxitin-resistant strains were detec-ted by ESBLs confirmatory test and AmpC-three-dimensional test ,respectively .The genotypes of ESBLs and AmpC β-lactamases were analyzed by PCR and sequence analysis .The virulence genes ( virA, ial, iapH, set1A, set1B and sen) in the cefoxitin-resistant strains were screened by PCR .Results Eight cefoxitin-re-sistant strains were identified from 356 Shigella isolates.Among them,six strains were identified as Shigella flexneri (S.fleaneri) strain (four F2a,one F2b and one F2c),and the rest were identified as Shigella sonnie (S.sonnei) strain.Among all eight cefoxitin-resistant strains,five strains showed positive results for ESBLs confirmatory test and two strains showed positive results for AmpC-three-dimensional test .Seven out of the eight strains harbor ESBLs genes (CTX-M-14,15,57 and/or OXA-30),two of which were positive for AmpC genes (DHA-1 and CMY-2).Five out of the eight cefoxitin-resistant strains carried all of the six tested viru-lence genes,while the other three strains possessed four virulence genes except for set1A and set1B.The two strains producing both ESBLs and AmpC β-lactamases were susceptible only to imipenem .Conclusion ESBLs positive isolates are prevalent strains of cefoxitin-resistant Shigella with potential high virulence .Some of them also produce AmpC β-lactamases ,and the DHA-1 type of AmpCβ-lactamase is identified for the first time in China .
