CAJ | 학술논문

目的：比较免疫双扩散法和速率散射比浊分析法在肺炎链球菌荚膜多糖抗原性评价方面的优劣。方法分别采用免疫双扩散法和速率散射比浊分析法检定来源于4个公司和美国ATCC的1型、6B型、9V型、10A型、14型和19A型肺炎链球菌荚膜多糖样品的抗原活性，同时比较肺炎链球菌免疫血清和仪器增益值对实验结果的影响。结果两种抗原活性检定方法都显示9V型4号样品的抗原活性缺失，其速率散射浊度信号值接近阴性对照；其余多糖样品均有沉淀线，速率散射浊度信号值高低不等；不同来源的肺炎链球菌抗血清和仪器增益值对速率散射比浊分析法的实验结果无明显干扰。结论速率散射比浊分析法能够定量分析各型肺炎链球菌荚膜多糖的抗原活性。
목적：비교면역쌍확산법화속솔산사비탁분석법재폐염련구균협막다당항원성평개방면적우렬。방법분별채용면역쌍확산법화속솔산사비탁분석법검정래원우4개공사화미국ATCC적1형、6B형、9V형、10A형、14형화19A형폐염련구균협막다당양품적항원활성，동시비교폐염련구균면역혈청화의기증익치대실험결과적영향。결과량충항원활성검정방법도현시9V형4호양품적항원활성결실，기속솔산사탁도신호치접근음성대조；기여다당양품균유침정선，속솔산사탁도신호치고저불등；불동래원적폐염련구균항혈청화의기증익치대속솔산사비탁분석법적실험결과무명현간우。결론속솔산사비탁분석법능구정량분석각형폐염련구균협막다당적항원활성。
Objective To comparatively analyze the advantages and disadvantages between a double immune-diffusion assay and a rate nephelometer analysis for the detection of antigen activity of pneu -mococcal capsular polysaccharides .Methods The antigen activity of pneumococcal capsular polysaccharides of serotypes 1,6B,9V,10A,14 and 19A from four manufacturers and ATCC were analyzed by a double im-mune-diffusion assay and a rate nephelometer analysis , respectively .The effects of antiserum samples and gain values on the rate response value were evaluated .Results The sample 4 of type 9V showed no antigeni-city with a rate response value similar to that of negative control as indicated by both tests .However ,the pre-cipitation lines and the rate response values presented by other polysaccharide samples differed in a wide range.Results of the rate nephelometer analysis were not affected by the anti -serum samples from different sources and the gain values .Conclusion The rate nephelometer analysis could quantitatively analyze the antigen activity of pneumococcal capsular polysaccharides .