浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
5期
393-395
,共3页
大鼠%脑卒中后抑郁%花椒多酚类化合物%NE%5-HT%MAO
大鼠%腦卒中後抑鬱%花椒多酚類化閤物%NE%5-HT%MAO
대서%뇌졸중후억욱%화초다분류화합물%NE%5-HT%MAO
rats%post-stroke depression%polyphenols extract from Zanthoxylum bungeanum Maxim%norepinephrine%5-hydroxytryptamine%monoamine oxidase
目的：探讨花椒多酚类化合物总提取物（ZPPC）抗脑卒中后抑郁作用机制。方法建立脑卒中后抑郁大鼠模型，酶联免疫法检测ZPPC、氟西汀对受试大鼠脑组织去甲肾上腺素（NE）、5-羟色胺（5-HT）含量的影响；采用荧光分光光度法测定ZPPC和吗氯贝胺对受试大鼠脑内单胺氧化酶（MAO）活性的影响。结果与空白对照组比较，模型组NE、5-HT水平显著降低（P约0.05），ZPPC高剂量组（200mg/kg）和氟西汀组与模型组比较，NE、5-HT水平均有显著性升高（P约0.05）；ZPPC中、高剂量组（100mg/kg和200mg/kg）还明显抑制大鼠脑组织MAO-A活性（P约0.05）。结论 ZPPC组能显著提高脑卒中后抑郁大鼠脑组织NE、5-HT水平，其作用机制可能与抑制脑组织MAO-A活性有关。
目的：探討花椒多酚類化閤物總提取物（ZPPC）抗腦卒中後抑鬱作用機製。方法建立腦卒中後抑鬱大鼠模型，酶聯免疫法檢測ZPPC、氟西汀對受試大鼠腦組織去甲腎上腺素（NE）、5-羥色胺（5-HT）含量的影響；採用熒光分光光度法測定ZPPC和嗎氯貝胺對受試大鼠腦內單胺氧化酶（MAO）活性的影響。結果與空白對照組比較，模型組NE、5-HT水平顯著降低（P約0.05），ZPPC高劑量組（200mg/kg）和氟西汀組與模型組比較，NE、5-HT水平均有顯著性升高（P約0.05）；ZPPC中、高劑量組（100mg/kg和200mg/kg）還明顯抑製大鼠腦組織MAO-A活性（P約0.05）。結論 ZPPC組能顯著提高腦卒中後抑鬱大鼠腦組織NE、5-HT水平，其作用機製可能與抑製腦組織MAO-A活性有關。
목적：탐토화초다분류화합물총제취물（ZPPC）항뇌졸중후억욱작용궤제。방법건립뇌졸중후억욱대서모형，매련면역법검측ZPPC、불서정대수시대서뇌조직거갑신상선소（NE）、5-간색알（5-HT）함량적영향；채용형광분광광도법측정ZPPC화마록패알대수시대서뇌내단알양화매（MAO）활성적영향。결과여공백대조조비교，모형조NE、5-HT수평현저강저（P약0.05），ZPPC고제량조（200mg/kg）화불서정조여모형조비교，NE、5-HT수평균유현저성승고（P약0.05）；ZPPC중、고제량조（100mg/kg화200mg/kg）환명현억제대서뇌조직MAO-A활성（P약0.05）。결론 ZPPC조능현저제고뇌졸중후억욱대서뇌조직NE、5-HT수평，기작용궤제가능여억제뇌조직MAO-A활성유관。
Objective To explore the mechanism of polyphenol extract from Zanthoxylum bungeanum Maxim. (ZPPC) on treating post-stroke depression. Methods Post-stroke depression model of rat was established. ELISA was used to detect the influence of ZPPC and fluoxetine on norepinephrine (NE) and 5-hydroxytryptamine (5-HT) contents in brain tissue and the monoamine oxidase (MAO) activity in rats brain was determined by fluorescence spectrophotometry. Results The levels of NE and 5-HT in brain tissue was significantly lower in model group as compared with control group (P<0.05), but dramatically elevated after treatment with high-dose ZPPC(200mg/kg) and fluoxetine (P<0.05). The activity of MAO in rats brain was inhibited by intervention of ZPPC with 100 and 200 mg/kg as compared with model group(P<0.05). Conclusion ZPPC can upregulate NE and 5-HT in brain tis-sue of rats with post-stroke depression and the underlying mechanism may involve inhibition of MAO activity.
