CAJ | 학술논문

目的：观察四君子汤联合缺血预处理（IPC）对肝脏缺血再灌注损伤大鼠炎症因子及氧化应激的影响。方法80只SD大鼠随机分成空白对照组、四君子汤预处理组、IPC组和四君子汤预处理+IPC组，每组20只。肝脏缺血再灌注术后检测白细胞计数（WBC）、血小板计数（PLT）；血清天门冬氨酸氨基转移酶（AST）、谷氨酸氨基转移酶（ALT）；血清肿瘤坏死因子α（TNF-α）、白介素6（IL-6），以及肝组织中丙二醛（MDA）和超氧化物歧化酶（SOD）水平。结果四君子汤预处理+IPC组WBC、ALT及AST水平显著低于其余三组（P约0.05）。四君子汤预处理组与IPC组的ALT、AST水平较空白对照组显著降低（P约0.05）。四君子汤预处理+IPC组TNF-α水平显著低于其余三组（P约0.05）。三个实验组IL-6、MDA水平显著低于空白对照组（P约0.05），SOD水平显著高于空白对照组（P约0.05）。各实验组间比较，四君子汤预处理+IPC组SOD水平高于其余两组（P约0.05）。结论四君子汤联合IPC可减轻大鼠肝脏缺血再灌注过氧化损伤，抑制炎症因子，保护肝功能。
목적：관찰사군자탕연합결혈예처리（IPC）대간장결혈재관주손상대서염증인자급양화응격적영향。방법80지SD대서수궤분성공백대조조、사군자탕예처리조、IPC조화사군자탕예처리+IPC조，매조20지。간장결혈재관주술후검측백세포계수（WBC）、혈소판계수（PLT）；혈청천문동안산안기전이매（AST）、곡안산안기전이매（ALT）；혈청종류배사인자α（TNF-α）、백개소6（IL-6），이급간조직중병이철（MDA）화초양화물기화매（SOD）수평。결과사군자탕예처리+IPC조WBC、ALT급AST수평현저저우기여삼조（P약0.05）。사군자탕예처리조여IPC조적ALT、AST수평교공백대조조현저강저（P약0.05）。사군자탕예처리+IPC조TNF-α수평현저저우기여삼조（P약0.05）。삼개실험조IL-6、MDA수평현저저우공백대조조（P약0.05），SOD수평현저고우공백대조조（P약0.05）。각실험조간비교，사군자탕예처리+IPC조SOD수평고우기여량조（P약0.05）。결론사군자탕연합IPC가감경대서간장결혈재관주과양화손상，억제염증인자，보호간공능。
Objective To investigate the effect of Sijunzi decoction and ischemia preconditioning (IPC) on in-flammatory mediators and oxidative stress in rats with hepatic ischemia-reperfusion. Methods Eighty SD rats were randomly divided into SHAM, Sijunzi decoction, IPC, and Sijunzi decoction+IPC groups(n=20 in each group). After hepatic ischemia-reperfusion, the levels of white blood cell count (WBC), platelet, alanine aminotransferase(ALT), aspartate aminotransferase(AST), tumor necrosis factor-alpha(TNF-α), interleukin-6(IL-6), and malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. Results The levels of WBC, ALT and AST in Sijunzi decoction+IPC group was significantly lower than those in other 3 groups (P<0.05). The levels of ALT and AST in Sijunzi decoction and IPC groups were significantly lower than those in sham group(P<0.05). The level of TNF-αin Sijunzi decoction+IPC group was significantly lower than that in other 3 groups (P<0.05). Compared with other 3 groups, the levels of IL-6 and MDA in sham group was significantly higher and SOD was significantly lower (Ps <0.05). The level of SOD in Sijunzi decoction+IPC group was significantly higher than that in Sijunzi decoction group and IPC group (P<0.05). Conclusion Sijunzi decoction combined with IPC can reduce hepatic ischemia-reperfusion injury in rats by alleviating inflammatory reaction, improving antioxidation capacity.