北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
5期
1-1,2
,共2页
胡椒%紫外分光光度法%胡椒碱
鬍椒%紫外分光光度法%鬍椒堿
호초%자외분광광도법%호초감
Pepper%Piperine%HPLC
目的：建立胡椒中胡椒碱的含量测定方法。方法：采用紫外分光光度法测定胡椒中胡椒碱的含量，用无水乙醇做空白，测定波长为342nm。结果：胡椒碱在0.85～6.80μg·ml-1范围内与吸光度呈良好的线性关系，回归方程为：A=0.0908x+0.0069，r=0.9998， r=0.9998。平均加样回收率为102.2%，RSD为1.03%。结论：该方法简便、准确、重复性好，可作为胡椒中胡椒碱的含量测定方法。
目的：建立鬍椒中鬍椒堿的含量測定方法。方法：採用紫外分光光度法測定鬍椒中鬍椒堿的含量，用無水乙醇做空白，測定波長為342nm。結果：鬍椒堿在0.85～6.80μg·ml-1範圍內與吸光度呈良好的線性關繫，迴歸方程為：A=0.0908x+0.0069，r=0.9998， r=0.9998。平均加樣迴收率為102.2%，RSD為1.03%。結論：該方法簡便、準確、重複性好，可作為鬍椒中鬍椒堿的含量測定方法。
목적：건립호초중호초감적함량측정방법。방법：채용자외분광광도법측정호초중호초감적함량，용무수을순주공백，측정파장위342nm。결과：호초감재0.85～6.80μg·ml-1범위내여흡광도정량호적선성관계，회귀방정위：A=0.0908x+0.0069，r=0.9998， r=0.9998。평균가양회수솔위102.2%，RSD위1.03%。결론：해방법간편、준학、중복성호，가작위호초중호초감적함량측정방법。
Objective:To establish a method for determination of piperine in pepper. Methods: using the UV to determination of piperine in pepper and anhydrous ethanol as blank, the detecting wavelength was at 342nm. Results:A good linearity of piperine was in the range of 0.85~6.8μg·ml-1,r=0.9998. The average recovery was 102.2% and RSD was 1.03%. Conclusion:This method is simple, accurate and with good reproducibility. It can be used for determination of piperine in pepper.
