特产研究
特產研究
특산연구
SPECIAL WILD ECONOMIC ANIMAL AND PLANT RESEARCH
2014年
1期
26-29
,共4页
布鲁氏杆菌%脂多糖%免疫原性
佈魯氏桿菌%脂多糖%免疫原性
포로씨간균%지다당%면역원성
Brucella%LPS%immunogenicity
采用热酚水法提取布鲁氏杆菌脂多糖，分离、纯化并SDS -PAGE银染鉴定。纯化的细菌内毒素免疫小鼠，通过斑点ELISA测定血清抗体效价，检测细菌内毒素免疫原性。采用热酚法能获得较高纯度的细菌内毒素，其免疫原性较高，斑点ELISA测血清效价达到1∶1000000，与全菌免疫原性接近。本研究为制备布鲁氏杆菌单克隆抗体和建立布鲁氏杆菌病快速诊断方法奠定研究基础。
採用熱酚水法提取佈魯氏桿菌脂多糖，分離、純化併SDS -PAGE銀染鑒定。純化的細菌內毒素免疫小鼠，通過斑點ELISA測定血清抗體效價，檢測細菌內毒素免疫原性。採用熱酚法能穫得較高純度的細菌內毒素，其免疫原性較高，斑點ELISA測血清效價達到1∶1000000，與全菌免疫原性接近。本研究為製備佈魯氏桿菌單剋隆抗體和建立佈魯氏桿菌病快速診斷方法奠定研究基礎。
채용열분수법제취포로씨간균지다당，분리、순화병SDS -PAGE은염감정。순화적세균내독소면역소서，통과반점ELISA측정혈청항체효개，검측세균내독소면역원성。채용열분법능획득교고순도적세균내독소，기면역원성교고，반점ELISA측혈청효개체도1∶1000000，여전균면역원성접근。본연구위제비포로씨간균단극륭항체화건립포로씨간균병쾌속진단방법전정연구기출。
The LPS was prepared from M5 Brucella in hot phenol water .With concentration ,enzymatic hydrolysis and alcohol precipitated to re-move impure protein .Then the purified LPS was identified by SDS -PAGE .The BALB/c mice were immunized with purified LPS as antigen to measure its immunogenicity .The serum antibody titers were detected with DOT -ELISA .The results showed that the LPS was highly purified and its immunogenicity was similar to the Brucella .The results lay the foundation for the preparation of monoclonal antibody and establishment of a rapid diagnostic method for Brucellosis .
