CAJ | 학술논문

在水温（25±2）℃下，给饲养在网箱（1.0m×0.5m×1.0m）中体质量（110±5）g的草鱼（Ctenopharyngodon idellus）投喂添加了金丝桃素（0、0.066%、0.132%）、绿原酸（0、0.04%、0.08%）和硬葡聚糖（0、0.2%、0.4%）饲料，以基础饲料组为对照组，探讨三种添加剂对草鱼幼鱼血清溶菌酶活性、白细胞吞噬率和血清补体C3含量的影响。结果表明：0.2%硬葡聚糖组草鱼溶菌酶活性最高，显著高于对照组（P＜0.05），饲喂35d后溶菌酶活性达到峰值，而金丝桃素和绿原酸对草鱼幼鱼血清溶菌酶活性没有显著性影响；饲料中添加0.08%绿原酸组草鱼血液中白细胞吞噬率和血清补体C3含量均显著高于对照组（P＜0.05），但与添加0.2%和0.4%硬葡聚糖组没有显著性差异（P＞0.05）；添加0.132%金丝桃素组的血清中补体C3含量在最低，且显著低于对照组（P＜0.05）。饲料中添加0.08%绿原酸和0.2%硬葡聚糖可有效增强草鱼幼鱼非特异免疫功能。
재수온（25±2）℃하，급사양재망상（1.0m×0.5m×1.0m）중체질량（110±5）g적초어（Ctenopharyngodon idellus）투위첨가료금사도소（0、0.066%、0.132%）、록원산（0、0.04%、0.08%）화경포취당（0、0.2%、0.4%）사료，이기출사료조위대조조，탐토삼충첨가제대초어유어혈청용균매활성、백세포탄서솔화혈청보체C3함량적영향。결과표명：0.2%경포취당조초어용균매활성최고，현저고우대조조（P＜0.05），사위35d후용균매활성체도봉치，이금사도소화록원산대초어유어혈청용균매활성몰유현저성영향；사료중첨가0.08%록원산조초어혈액중백세포탄서솔화혈청보체C3함량균현저고우대조조（P＜0.05），단여첨가0.2%화0.4%경포취당조몰유현저성차이（P＞0.05）；첨가0.132%금사도소조적혈청중보체C3함량재최저，차현저저우대조조（P＜0.05）。사료중첨가0.08%록원산화0.2%경포취당가유효증강초어유어비특이면역공능。
G rass carp (Ctenopharyngodon idellus)juveniles w ere fed on basaldiets supplem ented w ith differentlevels hypericin(0、0.066% and 0.132%),chlorogenic acid(0、0.04% and 0.08%)and scleroglucan(0、0.2% and 0.4%)for 42 days to evaluate the effects of the additives on lysozym e activity, phagocytic rate and com plem ent C 3 concentration in grass carp. T here w as significantly higher lysozym e activity in 0.2% scleroglucan group than that in the control group (P<0.05), the m axim al lysozym e activity in the 0.2%scleroglucan group on the 35th day.H ow ever,there w ere no significantincrease in the lysozym e activities in both hypericin groups and chlorogenicacidgroups(P>0.05).ThephagocyticrateandcomplementC3concentrationwereshowntobesignificantlyhigherin 0.04% chlorogenic acid group than those in the controlgroup (P<0.05).T here w ere no significantdifferences in the phagocytic rate and com plem ent C 3 concentration in 0.2% and 0.4% scleroglucan groups com pared to 0.08% chlorogenic acid group. T he m inim al complementC3concentrationwasfoundinthefishfedthedietcontaining0.132% hypericin,significantlylowerthanthatinthecontrol group (P<0.05).Thefindingsindicatethatthedietssupplementedwith0.08% ofchlorogenicacidor0.2% ofscleroglucanimprove the nonspecific im m une responses in grass carp juveniles.