CAJ | 학술논문

将性成熟的鲤鲫杂交鱼一代（F1）的雌鱼与雄性红鲫鱼杂交，获得了回交鲫子代，分析了平均体质量94.2g的1龄回交鲫子一代的细胞染色体核型，测定了其DNA含量。肾细胞直接制片法表明：回交鲫子代染色体由147条组成，即3n=147，NF=222，其核型公式为：3n=51m+24sm+27st+45t。流式细胞记数法结果表明：在20尾鱼中有14尾回交鲫子代细胞的DNA含量是对照鱼（红鲫鱼）的1.5倍，占总鱼数的70%；6尾在2～3倍体之间，非常接近三倍体，占总鱼数的30%。本结果与其细胞染色体核型分析结果基本一致，说明该鱼是以三倍体为主的回交种。
장성성숙적리즉잡교어일대（F1）적자어여웅성홍즉어잡교，획득료회교즉자대，분석료평균체질량94.2g적1령회교즉자일대적세포염색체핵형，측정료기DNA함량。신세포직접제편법표명：회교즉자대염색체유147조조성，즉3n=147，NF=222，기핵형공식위：3n=51m+24sm+27st+45t。류식세포기수법결과표명：재20미어중유14미회교즉자대세포적DNA함량시대조어（홍즉어）적1.5배，점총어수적70%；6미재2～3배체지간，비상접근삼배체，점총어수적30%。본결과여기세포염색체핵형분석결과기본일치，설명해어시이삼배체위주적회교충。
The karyotypes were observed in metaphase chromo some specimens of back cross crucian carp F1 bred by female hybrids of common carp and crucian carp crossed with male crucian carp and injected with PHA and colchicine by short-term culture of kidney cells with air-drying technique.The DNA content sweredeter mined in the somaticcells of the backcross cru-cian carp F1 with body weight of 94. 2g using flowing cytometer and the DNA content of diploid control of red crucian carp as standard.The backcross cru-cian carp F1 had chromosome number of 3n= 147, with the karyotype form ula as 3n= 51m+ 24sm+ 27st+ 45t, and arm number of NF=222.The DNA relative contents of fourteen individuals of the backcross crucian carp F1,representing 70% of the twenty samples, were found to beashighas 1.5times of that in the diploidcontrol fish with.There were 6 triploid in dividualsaccounting for 30% of the total fish,showing the consistent with the results of the cellularkaryotypeanalysis,thatis,the backcross crucian carp F1 being the progeny with triploid cells.