CAJ | 학술논문

目的：观察淫羊藿苷( icariin,ICA)对自发性高血压大鼠肾间质纤维化的干预作用,并探讨其机制。方法14只13周龄的♂自发性高血压大鼠(SHR),随机分为模型组(n=7)和ICA组(n=7),同源正常的京都大鼠(WKY)作为对照组(n=7)。所有大鼠适应性饲养1周后,ICA组给予ICA 40 mg·kg-1,ig,bid,至26周龄,模型组和对照组给予等量生理盐水。通过HE和Masson染色观察肾脏病理学变化；双抗体夹心法测定大鼠血浆醛固酮和Ⅲ型胶原含量；real time RT-PCR 法检测肾脏组织中转化生长因子-β1( transfor-ming growth factor-β1,TGF-β1)、Smad2、结缔组织生长因子( connective tissue growth factor, CTGF )、纤维连接蛋白( fi-bronectin,FN) mRNA的表达。结果与正常对照组比较,模型组肾脏结构较紊乱,肾小球系膜基质增多,肾小管间质纤维化明显；血浆醛固酮和Ⅲ型胶原含量增高( P <0．01)；TGF-β1、Smad2、CTGF、FN mRNA的表达明显上调( P<0．01或P<0．05)。与模型组比较,ICA组肾脏结构有所改善,纤维化减轻；血浆醛固酮和Ⅲ型胶原含量降低( P<0．01或P<0．05)；TGF-β1、Smad2、CTGF、FN mRNA的表达明显下调(P<0．01或P<0．05)。结论 ICA能改善自发性高血压大鼠肾间质纤维化,其机制可能与降低血浆醛固酮水平及下调TGF-β1和 Smad2的表达有关。
목적：관찰음양곽감( icariin,ICA)대자발성고혈압대서신간질섬유화적간예작용,병탐토기궤제。방법14지13주령적♂자발성고혈압대서(SHR),수궤분위모형조(n=7)화ICA조(n=7),동원정상적경도대서(WKY)작위대조조(n=7)。소유대서괄응성사양1주후,ICA조급여ICA 40 mg·kg-1,ig,bid,지26주령,모형조화대조조급여등량생리염수。통과HE화Masson염색관찰신장병이학변화；쌍항체협심법측정대서혈장철고동화Ⅲ형효원함량；real time RT-PCR 법검측신장조직중전화생장인자-β1( transfor-ming growth factor-β1,TGF-β1)、Smad2、결체조직생장인자( connective tissue growth factor, CTGF )、섬유련접단백( fi-bronectin,FN) mRNA적표체。결과여정상대조조비교,모형조신장결구교문란,신소구계막기질증다,신소관간질섬유화명현；혈장철고동화Ⅲ형효원함량증고( P <0．01)；TGF-β1、Smad2、CTGF、FN mRNA적표체명현상조( P<0．01혹P<0．05)。여모형조비교,ICA조신장결구유소개선,섬유화감경；혈장철고동화Ⅲ형효원함량강저( P<0．01혹P<0．05)；TGF-β1、Smad2、CTGF、FN mRNA적표체명현하조(P<0．01혹P<0．05)。결론 ICA능개선자발성고혈압대서신간질섬유화,기궤제가능여강저혈장철고동수평급하조TGF-β1화 Smad2적표체유관。
Aim To investigate the effect of icariin ( ICA) on renal interstitial fibrosis in SHR and explore its mechanism. Methods Fourteen male SHR of 13-week-old were randomly divided into model group( n=7 ) and ICA group ( n=7 ) , and WKY as control group (n=7). One week after adaptive breeding,the rats in the ICA group were given ICA 40 mg·kg-1,ig,bid to 26-week-old. The other rats in the model group and control group were given the same amount of normal sa-line. Then, the morphological changes of the kidney were observed by HE and Masson staining,respective-ly. The contents of plasma aldosterone andⅢcollagen were measured by double antibody sandwich method. The mRNA expressions of TGF-β1 , Smad2 , CTGF and FN were examined by real time RT-PCR. Results Compared with the normal control group, the kidney structures of model group were disordered,the mesang-ial matrix and the tubular interstitial fibrosis were in-creased. The contents of plasma aldosterone and Ⅲcollagen were increased in model group ( P <0. 01 ) . And the mRNA expressions of TGF-β1 , Smad2 , CTGF and FN in kidney tissues were up-regulated in model group( P <0. 01 or P <0. 05 ) . Compared with the model group,the kidney structures were improved and the contents of plasma aldosterone and Ⅲ collagen were reduced, as well as the mRNA expressions of TGF-β1,Smad2,CTGF and FN in kidney tissues were down-regulated(P<0. 01 or P<0. 05)in ICA group. Conclusion ICA may have anti-renal interstitial fi-brosis effect on SHR,and the mechanism might be re-lated to the reduced plasma aldosterone levels and the down-regulated expression of TGF-β1 and Smad2 .