世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
2期
329-334
,共6页
王俊%刘霞%张雅琴%宋明%林韵涵%马孝煕%孙伟%向丽%胡志刚%邬兰%张雪琼%胡伟毅
王俊%劉霞%張雅琴%宋明%林韻涵%馬孝煕%孫偉%嚮麗%鬍誌剛%鄔蘭%張雪瓊%鬍偉毅
왕준%류하%장아금%송명%림운함%마효희%손위%향려%호지강%오란%장설경%호위의
苍耳子%DNA条形码%ITS2%鉴定
蒼耳子%DNA條形碼%ITS2%鑒定
창이자%DNA조형마%ITS2%감정
Xanthii Fructus%DNA barcoding%ITS2%identification
目的：应用 ITS2序列快速并准确地鉴定中药材苍耳子，为其药材质量、用药安全提供保障。方法：提取苍耳子药材及其混伪品的基因组DNA、扩增 ITS2序列并测序，采用软件CodonCode Aligner V 4.2对测序峰图进行校对拼接，并对峰图进行质量控制。应用MEGA 5.0软件计算种内种间 Kimura 2-parameter （K2P）遗传距离,构建邻接（NJ）系统聚类树。结果：苍耳子药材基原物种种内 K2P 遗传距离为0，药材基原物种与其他混伪品的 K2P遗传距离分布于0.009~0.542；NJ树结果显示苍耳子药材与其混伪品均可明显区分。结论：ITS2序列适用于中药材苍耳子及其混伪品鉴别，进一步验证了DNA条形码技术鉴定中药材的有效性。
目的：應用 ITS2序列快速併準確地鑒定中藥材蒼耳子，為其藥材質量、用藥安全提供保障。方法：提取蒼耳子藥材及其混偽品的基因組DNA、擴增 ITS2序列併測序，採用軟件CodonCode Aligner V 4.2對測序峰圖進行校對拼接，併對峰圖進行質量控製。應用MEGA 5.0軟件計算種內種間 Kimura 2-parameter （K2P）遺傳距離,構建鄰接（NJ）繫統聚類樹。結果：蒼耳子藥材基原物種種內 K2P 遺傳距離為0，藥材基原物種與其他混偽品的 K2P遺傳距離分佈于0.009~0.542；NJ樹結果顯示蒼耳子藥材與其混偽品均可明顯區分。結論：ITS2序列適用于中藥材蒼耳子及其混偽品鑒彆，進一步驗證瞭DNA條形碼技術鑒定中藥材的有效性。
목적：응용 ITS2서렬쾌속병준학지감정중약재창이자，위기약재질량、용약안전제공보장。방법：제취창이자약재급기혼위품적기인조DNA、확증 ITS2서렬병측서，채용연건CodonCode Aligner V 4.2대측서봉도진행교대병접，병대봉도진행질량공제。응용MEGA 5.0연건계산충내충간 Kimura 2-parameter （K2P）유전거리,구건린접（NJ）계통취류수。결과：창이자약재기원물충충내 K2P 유전거리위0，약재기원물충여기타혼위품적 K2P유전거리분포우0.009~0.542；NJ수결과현시창이자약재여기혼위품균가명현구분。결론：ITS2서렬괄용우중약재창이자급기혼위품감별，진일보험증료DNA조형마기술감정중약재적유효성。
Objective: To identify Xanthii Fructus and secure its quality and safety in medication. Methods: Total ge-nomic DNA was extracted from Xanthii Fructus and its adulterants. ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V 4.2. The Kimura 2-Parameter (K2P) distances were calculated using MEGA 5.0. The neigh-bor-joining (NJ) phylogenetic trees were constructed. Results: The intraspecific genetic distances of Xanthii Fructus were 0. The interspecific genetic distances between Xanthii Fructus and its adulterants were ranged from 0.009 to 0.542. The NJ tree showed that Xanthii Fructus could differ from its adulterants obviously. Conclusion: ITS2 can be used to identify Xanthii Fructus from its adulterants effectively, and our study further confirmed the effectiveness of ITS2 to identify traditional Chinese medicinal materials.
