CAJ | 학술논문

目的：本研究采用形态学与DNA条形码技术，建立维吾尔药材新疆圆柏Juniperus sabina L.及其混伪品的鉴别方法。方法：随机选择不同产地新疆圆柏及其混伪品的叶片和果实，比较外观差异；采用ITS2序列对新疆圆柏及其混伪品10份样品进行PCR扩增并测序，结合GenBank获得4条侧柏ITS2序列，比较14份样品ITS2序列种内和种间变异，采用K2P模型构建NJ系统树。结果：4种植物叶片和果实外观相态均有相似之处；DNA条形码ITS2序列鉴定结果显示，14份研究样品ITS2序列长度均为219 bp，种间最大K2P距离为0.089。结论：ITS2序列及NJ树可将新疆圆柏与3种混伪品植物区分，为维吾尔药材新疆圆柏的标准化奠定基础。
목적：본연구채용형태학여DNA조형마기술，건립유오이약재신강원백Juniperus sabina L.급기혼위품적감별방법。방법：수궤선택불동산지신강원백급기혼위품적협편화과실，비교외관차이；채용ITS2서렬대신강원백급기혼위품10빈양품진행PCR확증병측서，결합GenBank획득4조측백ITS2서렬，비교14빈양품ITS2서렬충내화충간변이，채용K2P모형구건NJ계통수。결과：4충식물협편화과실외관상태균유상사지처；DNA조형마ITS2서렬감정결과현시，14빈연구양품ITS2서렬장도균위219 bp，충간최대K2P거리위0.089。결론：ITS2서렬급NJ수가장신강원백여3충혼위품식물구분，위유오이약재신강원백적표준화전정기출。
To establish the DNA barcode identification method of Uygur Medicine Juniperus sabina. Leaves and fruits were selected randomly from different areas to contrast morphology; Ten samples were amplified by ITS2 se-quence, combining with four ITS sequences of Platycladus orientalis obtained from GenBank, intraspecific and inter-specific variation were compared, K2P and NJ methods were utilized for analysising the relationship. The results show J. sabina cannot be distinguished with its adulterants by morphological characteristics of leaves and fruits; 14 Samples of ITS2 sequence length are 219 bp, the maximum K2P distance are 0.089. J. sabina and its 3 adulterants plant can be distinguished by the DNA barcode ITS2 sequence and NJ tree successfully. Therefore, ITS2 barcode sequence was able to identify J. sabina and its adulterants species, which provides an effective way for the molecular identification of Uygur medicine J. sabina.