CAJ | 학술논문

背景与目的研究聚集相关蛋白（accumulation-associated protein, Aap）基因及转化生长因子β1（TGF-β1）对肺癌相关生物材料表皮葡萄球菌（SE）生物膜形成的影响。方法种属鉴定分离临床肺癌患者植入材料感染表皮葡萄球菌株，PCR法检测生物膜形成相关基因Aap，检测表皮葡萄球菌Aap基因株生物形成能力。密度梯度法提取非小细胞肺癌患者外周血单个核细胞，与人肺腺癌细胞A549在不同浓度（10 ng/mL,20 ng/mL,40 ng/mL） TGF-β1共培养30 h后，取上清液分别加入SE Aap+株、SE Aap-株下与医用硅橡胶培养30 h，半定量粘附试验测各组细菌生物膜形成的情况，扫描电镜观察材料表面生物膜微观情况。结果 Aap基因的与表皮葡萄球菌生物膜的形成密切相关（P<0.01）。在（10 ng/mL,20 ng/mL,40 ng/mL）TGF-β1浓度组医用硅橡胶表面SE Aap+生物膜的厚度大于空白组（P<0.01）。SE Aap+株在TGF-β1浓度组间生物膜的厚度无明显差异（P>0.05）。在不同浓度TGF-β1因子刺激下SE Aap-株均不能形成明显细菌生物膜。结论在肺癌患者植入材料引起感染中表皮葡萄球菌Aap基因表达阳性株较易形成细菌生物膜，TGF-β1对SE Aap阳性形成细菌生物膜有一定促进作用。
배경여목적연구취집상관단백（accumulation-associated protein, Aap）기인급전화생장인자β1（TGF-β1）대폐암상관생물재료표피포도구균（SE）생물막형성적영향。방법충속감정분리림상폐암환자식입재료감염표피포도구균주，PCR법검측생물막형성상관기인Aap，검측표피포도구균Aap기인주생물형성능력。밀도제도법제취비소세포폐암환자외주혈단개핵세포，여인폐선암세포A549재불동농도（10 ng/mL,20 ng/mL,40 ng/mL） TGF-β1공배양30 h후，취상청액분별가입SE Aap+주、SE Aap-주하여의용규상효배양30 h，반정량점부시험측각조세균생물막형성적정황，소묘전경관찰재료표면생물막미관정황。결과 Aap기인적여표피포도구균생물막적형성밀절상관（P<0.01）。재（10 ng/mL,20 ng/mL,40 ng/mL）TGF-β1농도조의용규상효표면SE Aap+생물막적후도대우공백조（P<0.01）。SE Aap+주재TGF-β1농도조간생물막적후도무명현차이（P>0.05）。재불동농도TGF-β1인자자격하SE Aap-주균불능형성명현세균생물막。결론재폐암환자식입재료인기감염중표피포도구균Aap기인표체양성주교역형성세균생물막，TGF-β1대SE Aap양성형성세균생물막유일정촉진작용。
Background and objective This study was conducted to evaluate the effects of the accumulation-associated protein (Aap) gene and transform growth factor-beta 1 (TGF-β1) on the bioiflm formation of lung cancer-related Staphylococcus epidermidis (SE). Methods Species identification was performed to isolate SE strains from clinically im-planted materials in lung cancer patients. Stable genetic aggregated proteins, which are associated with negative and positive isolates, were obtained. hTe bioiflm-formation ability of the SE Aap gene was determined by PCR. Density gradient method was used to extract peripheral blood mononuclear cells from patients with non-small cell lung cancer. Atfer 30 h, these cells were co-cultured with A549 at different TGF-β1 concentrations. hTe supernatant was then combined with SE Aap+and SE Aap-strains and co-cultured with a medical silicone rubber. A semi-quantitative adhesion test was performed for each bacterial bioiflm formation. Scanning electron microscopy was also conducted to observe the microcosmic condition of this material on the bacterial bioiflm surface. Results hTe Aap gene was closely related to SE bioiflm formation. At 10 ng/mL, 20 ng/mL, and 40 ng/mL, SE Aap+bioiflm on the medical silicone rubber surface was thicker in the TGF-β1 group than in the control group. No signiifcant differences were found between TGF-β1 groups. For the SE Aap-strains, no evident bioiflm was formed in TGF-β1 and control groups. Conclusion In plant material-related infection of lung cancer patients, SE Aap+strain easily forms bioiflm. Furthermore, TGF-β1 was conducive for the bioiflm formation of SE Aap+strains.