CAJ | 학술논문

目的：为建立基于单核苷酸多态性（SNP）技术鉴别砂仁3个基原（阳春砂A momum villosum、海南砂A momum longiligulare、绿壳砂A momum villosum var. xanthioides）的方法，对这3个种共计60份材料ITS2序列进行分析比较。方法：将实验所得砂仁ITS2序列，应用CodonCode Aligner 软件进行序列拼接比对。用MEGA 5.0导出各序列变异位点，并对变异位点进行分析。为验证结果准确性，下载砂仁3个基原GenBank的所有ITS2序列，共计34条，对结果进行验证。结果：砂仁ITS2序列比对后长度为230 bp，3个不同基原在135 bp和199 bp处存在稳定SNP（s）变异位点。结论：本研究开发的两个稳定的SNP（s）位点可以快速准确地鉴定砂仁药材3个基原。
목적：위건립기우단핵감산다태성（SNP）기술감별사인3개기원（양춘사A momum villosum、해남사A momum longiligulare、록각사A momum villosum var. xanthioides）적방법，대저3개충공계60빈재료ITS2서렬진행분석비교。방법：장실험소득사인ITS2서렬，응용CodonCode Aligner 연건진행서렬병접비대。용MEGA 5.0도출각서렬변이위점，병대변이위점진행분석。위험증결과준학성，하재사인3개기원GenBank적소유ITS2서렬，공계34조，대결과진행험증。결과：사인ITS2서렬비대후장도위230 bp，3개불동기원재135 bp화199 bp처존재은정SNP（s）변이위점。결론：본연구개발적량개은정적SNP（s）위점가이쾌속준학지감정사인약재3개기원。
Medicinal plants of the Fructus Amomi containing three species (A momum villosum, A momum longiligu-lare, Amomum villosum var. xanthioides)are well-known, which are widely used as traditional medicines. The mor-phological characteristics of the three origins are very similar, especially in the form of seed. In this study, 60 sam-ples of Fructus Amomi were co llected, and 34 sequences of the Fructus Amomi and their adulterants from GenBank were analyzed. Single nucleotide polymorphisms (SNPs) were detected. All the ITS2 sequences here (including our ex-periments and GenBank data)were examined for SNPs at the interspecies level. Results from the study revealed that two stable bases at position 135 bp and 199 bp were found, which could be used as a unique marker to distinguish the three origins of Fructus Amomi. The two SNPs in the ITS2 were found to exist stably between the three species, and all the GenBank sequences of the Fructus Amomi. Our findings indicated that SNP-based DNA barcoding could be used as an efficient method for the rapid and accurate identification of the three origins of Fructus Amomi.