CAJ | 학술논문

目的：对中药材蛇蜕及其易混伪品进行分子鉴定，以确保该中药材的临床用药安全。方法：以COI序列作为DNA条形码，对蛇蜕原药材进行DNA提取，PCR扩增和序列测定，对13个物种的68份样品进行DNA Barcoding Gap分析和系统聚类分析。结果：蛇蜕3种基原黑眉锦蛇 Elap he tae niura Cope、锦蛇Elap he c arinata（Guenther）和乌梢蛇 Zaoc ys dhumnade s（Cantor）具有DNA Barcoding Gap ，在邻接（NJ）系统聚类树上分别聚为独立一枝。结论：COI作为DNA条形码，不仅可以鉴定中药材蛇蜕的3种基原，而且可以区分蛇蜕及其易混伪品，表明DNA条形码可以用于中药材蛇蜕的鉴定。
목적：대중약재사세급기역혼위품진행분자감정，이학보해중약재적림상용약안전。방법：이COI서렬작위DNA조형마，대사세원약재진행DNA제취，PCR확증화서렬측정，대13개물충적68빈양품진행DNA Barcoding Gap분석화계통취류분석。결과：사세3충기원흑미금사 Elap he tae niura Cope、금사Elap he c arinata（Guenther）화오소사 Zaoc ys dhumnade s（Cantor）구유DNA Barcoding Gap ，재린접（NJ）계통취류수상분별취위독립일지。결론：COI작위DNA조형마，불부가이감정중약재사세적3충기원，이차가이구분사세급기역혼위품，표명DNA조형마가이용우중약재사세적감정。
Objective: This study aimed to distinguish Serpentis Periostracum from its adulterants, which will provide the basis for its safe application. Methods: Here, COI sequences of 68 samples from 13 species were PCR amplified and sequenced. Furthermore, the DNA Barcoding Gap and phylogenetic cluster analysis were carried out. Results:The results exhibited that the COI sequences of all the three origin animals of Serpentis Periostracum have DNA Barcoding Gap. For phylogenetic cluster analysis, all the three origin animals showed monophyletic and every species can be discriminated clearly. Conclusion: COI is an effective DNA barcode for the identification of Serpen-tis Periostracum.