CAJ | 학술논문

采用高效液相-四级杆飞行时间质谱联用技术（HPLC-QTOF/MS）建立了冬虫夏草高效液相特征图谱。色谱条件为Agilent Zorbax SB Aq（150 mm×4.6 mm，5μm）色谱柱；流动相为5 mmol·L-1乙酸铵和乙腈，梯度洗脱；流速0.8 mL·min-1；检测波长260 nm。该方法分别从冬虫夏草中鉴定了9个化学成分，冬虫夏草无性型中华被毛孢菌丝体中鉴定了9个化学成分，北冬虫夏草中鉴定了8个化学成分，成功运用于3种虫草样品分析。
채용고효액상-사급간비행시간질보련용기술（HPLC-QTOF/MS）건립료동충하초고효액상특정도보。색보조건위Agilent Zorbax SB Aq（150 mm×4.6 mm，5μm）색보주；류동상위5 mmol·L-1을산안화을정，제도세탈；류속0.8 mL·min-1；검측파장260 nm。해방법분별종동충하초중감정료9개화학성분，동충하초무성형중화피모포균사체중감정료9개화학성분，북동충하초중감정료8개화학성분，성공운용우3충충초양품분석。
A HPLC-QTOF MS method was established for analysis of components in Ophiocordyceps sinensis . The HPLC analysis was performed on an Agilent Zorbax SB Aq (150 mmí4.6 mm, 5 μm) with gr adient elution (5 mmol·L-1 ammonium acetate aqueous solution-acetonitrile), flow rate was 0.8 mL·min-1 and detection wave-length was 260 nm. The developed method was successfully applied in analysis of three different samples in-cluding O. sinensis, Hirsutella sinensis ( anamorph of O.sinensis) and Cordyceps militaris. Nine compounds were i-dentified in both O.sinensis and H.sinensis, which eight compounds were identified in C.militaris.